MAC-1 marks a quiescent and functionally superior HSC subset during regeneration

Anna Rydström, Els Mansell, Valgardur Sigurdsson, Julia Sjöberg, Shamit Soneji, Kenichi Miharada, Jonas Larsson

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Mouse hematopoietic stem cells (HSCs) have been extensively defined both molecularly and functionally at steady state, while regenerative stress induces immunophenotypical changes that limit high purity isolation and analysis. It is therefore important to identify markers that specifically label activated HSCs to gain further knowledge about their molecular and functional properties. Here, we assessed the expression of macrophage-1 antigen (MAC-1) on HSCs during regeneration following transplantation and observed a transient increase in MAC-1 expression during the early reconstitution phase. Serial transplantation experiments demonstrated that reconstitution potential was highly enriched in the MAC-1+ portion of the HSC pool. Moreover, in contrast to previous reports, we found that MAC-1 expression inversely correlates with cell cycling, and global transcriptome analysis showed that regenerating MAC-1+ HSCs share molecular features with stem cells with low mitotic history. Taken together, our results suggest that MAC-1 expression marks predominantly quiescent and functionally superior HSCs during early regeneration.

Original languageEnglish
Pages (from-to)736-748
Number of pages13
JournalStem Cell Reports
Volume18
Issue number3
DOIs
Publication statusPublished - 14 Mar 2023
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

Fingerprint

Dive into the research topics of 'MAC-1 marks a quiescent and functionally superior HSC subset during regeneration'. Together they form a unique fingerprint.

Cite this