Madurella mycetomatis grains within a eumycetoma lesion are clonal

Bertrand Nyuykonge, Emmanuel Edwar Siddig, Mickey Konings, Sahar Bakhiet, Annelies Verbon, Corné H.W. Klaassen, Ahmed Hassan Fahal, Wendy W.J. Van De Sande*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Downloads (Pure)


Eumycetoma is a neglected tropical infection of the subcutaneous tissue, characterized by tumor-like lesions and most commonly caused by the fungus Madurella mycetomatis. In the tissue, M. mycetomatis organizes itself in grains, and within a single lesion, thousands of grains can be present. The current hypothesis is that all these grains originate from a single causative agent, however, this hypothesis was never proven. Here, we used our recently developed MmySTR assay, a highly discriminative typing method, to determine the genotypes of multiple grains within a single lesion. Multiple grains from surgical lesions obtained from 11 patients were isolated and genotyped using the MmySTR panel. Within a single lesion, all tested grains shared the same genotype. Only in one single grain from one patient, a difference of one repeat unit in one MmySTR marker was noted relative to the other grains from that patient. We conclude that within these lesions the grains originate from a single clone and that the inherent unstable nature of the microsatellite markers may lead to small genotypic differences. Lay Abstract: In lesions of the implantation mycosis mycetoma many Madurella mycetomatis grains are noted. It was unknown if grains arose after implantation of a single isolate or a mixture of genetically diverse isolates. By typing the mycetoma grains we showed that all grains within a single lesion were clonal and originated from a single isolate.

Original languageEnglish
Article numbermyac051
JournalMedical Mycology
Issue number7
Publication statusPublished - Jul 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s). Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.


Dive into the research topics of 'Madurella mycetomatis grains within a eumycetoma lesion are clonal'. Together they form a unique fingerprint.

Cite this