Magnetic Resonance Detection of CD34(+) Cells from Umbilical Cord Blood Using a F-19 Label

Lucia Duinhouwer, BJM van Rossum, Sandra van Tiel, Ramon Werf, Gabriela Doeswijk, Joost Haeck, EWJC Rombouts, Mariëtte ter Borg, Gyula Kotek, Eric Braakman, Jan Cornelissen, Monique Bernsen

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Abstract

Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34(+) cells, with F-19-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34(+) cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.
Original languageUndefined/Unknown
JournalPLoS One (print)
Volume10
Issue number9
DOIs
Publication statusPublished - 2015

Research programs

  • EMC MM-01-40-01
  • EMC MM-02-41-04
  • EMC NIHES-03-30-03

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