Magnetic Resonance Detection of CD34(+) Cells from Umbilical Cord Blood Using a F-19 Label

Lucia Duinhouwer; BJM van Rossum; Sandra van Tiel; Ramon Werf; Gabriela Doeswijk; Joost Haeck; EWJC Rombouts; Mariëtte ter Borg; Gyula Kotek; Eric Braakman; Jan Cornelissen; Monique Bernsen

doi:10.1371/journal.pone.0138572

Magnetic Resonance Detection of CD34(+) Cells from Umbilical Cord Blood Using a F-19 Label

Lucia Duinhouwer, BJM van Rossum, Sandra van Tiel, Ramon Werf, Gabriela Doeswijk, Joost Haeck, EWJC Rombouts, Mariëtte ter Borg, Gyula Kotek, Eric Braakman, Jan Cornelissen, Monique Bernsen

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34(+) cells, with F-19-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34(+) cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.

Original language	Undefined/Unknown
Journal	PLoS One (print)
Volume	10
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0138572
Publication status	Published - 2015

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10.1371/journal.pone.0138572

2015-12-07-042.pdfFinal published version, 2.34 MB

http://hdl.handle.net/1765/79205

Cite this

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title = "Magnetic Resonance Detection of CD34(+) Cells from Umbilical Cord Blood Using a F-19 Label",

abstract = "Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34(+) cells, with F-19-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34(+) cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.",

author = "Lucia Duinhouwer and {van Rossum}, BJM and {van Tiel}, Sandra and Ramon Werf and Gabriela Doeswijk and Joost Haeck and EWJC Rombouts and {ter Borg}, Mari{\"e}tte and Gyula Kotek and Eric Braakman and Jan Cornelissen and Monique Bernsen",

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T1 - Magnetic Resonance Detection of CD34(+) Cells from Umbilical Cord Blood Using a F-19 Label

AU - Duinhouwer, Lucia

AU - van Rossum, BJM

AU - van Tiel, Sandra

AU - Werf, Ramon

AU - Doeswijk, Gabriela

AU - Haeck, Joost

AU - Rombouts, EWJC

AU - ter Borg, Mariëtte

AU - Kotek, Gyula

AU - Braakman, Eric

AU - Cornelissen, Jan

AU - Bernsen, Monique

PY - 2015

Y1 - 2015

N2 - Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34(+) cells, with F-19-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34(+) cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.

AB - Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34(+) cells, with F-19-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34(+) cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.

U2 - 10.1371/journal.pone.0138572

DO - 10.1371/journal.pone.0138572

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