MicroRNA-155 controls T helper cell activation during viral infection

Eliana Goncalves-Alves, Victoria Saferding, Christopher Schliehe, Robert Benson, Mariola Kurowska-Stolarska, Julia Stefanie Brunner, Antonia Puchner, Bruno K. Podesser, Josef S. Smolen, Kurt Redlich, Michael Bonelli, James Brewer, Andreas Bergthaler, Günter Steiner, Stephan Blüml*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)
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Abstract

MicroRNA (miR) 155 has been implicated in the regulation of innate and adaptive immunity as well as autoimmune processes. Importantly, it has been shown to regulate several antiviral responses, but its contribution to the immune response against cytopathic viruses such as vesicular stomatitis virus (VSV) infections is not known. Using transgenic/recombinant VSV expressing ovalbumin, we show that miR-155 is crucially involved in regulating the T helper cell response against this virus. Our experiments indicate that miR-155 in CD4+ T cells controls their activation, proliferation, and cytokine production in vitro and in vivo upon immunization with OVA as well as during VSV viral infection. Using intravital multiphoton microscopy we analyzed the interaction of antigen presenting cells (APCs) and T cells after OVA immunization and found impaired complex formation when using miR-155 deficient CD4+ T cells compared to wildtype CD4+ T cells ex vivo. In contrast, miR-155 was dispensable for the maturation of myeloid APCs and for their T cell stimulatory capacity. Our data provide the first evidence that miR-155 is required for efficient CD4+ T cell activation during anti-viral defense by allowing robust APC-T cell interaction required for activation and cytokine production of virus specific T cells.

Original languageEnglish
Article number1367
Number of pages10
JournalFrontiers in Immunology
Volume10
Issue numberJUN
DOIs
Publication statusPublished - 13 Jun 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2019 Goncalves-Alves, Saferding, Schliehe, Benson, Kurowska-Stolarska, Brunner, Puchner, Podesser, Smolen, Redlich, Bonelli, Brewer, Bergthaler, Steiner and Blüml. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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