TY - JOUR
T1 - Minimal information for studies of extracellular vesicles (MISEV2023)
T2 - From basic to advanced approaches
AU - Welsh, Joshua A.
AU - Goberdhan, Deborah C. I.
AU - O'Driscoll, Lorraine
AU - MISEV Consortium
AU - Buzas, Edit I.
AU - Blenkiron, Cherie
AU - Bussolati, Benedetta
AU - Cai, Houjian
AU - Di Vizio, Dolores
AU - Driedonks, Tom A. P.
AU - Erdbrugger, Uta
AU - Falcon-Perez, Juan M.
AU - Fu, Qing-Ling
AU - Hill, Andrew F.
AU - Lenassi, Metka
AU - Lim, Sai Kiang
AU - Mahoney, My G.
AU - Mohanty, Sujata
AU - Moller, Andreas
AU - Nieuwland, Rienk
AU - Ochiya, Takahiro
AU - Sahoo, Susmita
AU - Torrecilhas, Ana C.
AU - Zheng, Lei
AU - Zijlstra, Andries
AU - Abuelreich, Sarah
AU - Bagabas, Reem
AU - Bergese, Paolo
AU - Bridges, Esther M.
AU - Brucale, Marco
AU - Burger, Dylan
AU - Carney, Randy P.
AU - Cocucci, Emanuele
AU - Colombo, Federico
AU - Crescitelli, Rossella
AU - Hanser, Edveena
AU - Harris, Adrian L.
AU - Haughey, Norman J.
AU - Hendrix, An
AU - Ivanov, Alexander R.
AU - Jovanovic-Talisman, Tijana
AU - Kruh-Garcia, Nicole A.
AU - Faustino, Vroniqa Ku'ulei-Lyn
AU - Kyburz, Diego
AU - Lasser, Cecilia
AU - Lennon, Kathleen M.
AU - Lotvall, Jan
AU - Maddox, Adam L.
AU - Martens-Uzunova, Elena S.
AU - Mizenko, Rachel R.
AU - Newman, Lauren A.
AU - van Royen, Martin
N1 - Publisher Copyright:
© 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
PY - 2024/2/1
Y1 - 2024/2/1
N2 - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
AB - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
UR - https://www.scopus.com/pages/publications/85183359756
U2 - 10.1002/jev2.12404
DO - 10.1002/jev2.12404
M3 - Article
C2 - 38326288
SN - 2001-3078
VL - 13
SP - e12404
JO - Journal of Extracellular Vesicles
JF - Journal of Extracellular Vesicles
IS - 2
M1 - e12404
ER -