MLPA for prenatal diagnosis of commonly occurring aneuploidies

Jan Schouten, Robert-Jan Galjaard

Research output: Chapter/Conference proceedingChapterAcademic

8 Citations (Scopus)


Multiplex ligation-dependent probe amplification (MLPA) is a new method to
determine the copy number of up to 45 genomic DNA sequences in a single multiplex
polymerase chain reaction (PCR)-based reaction. In contrast to standard multiplex PCR,
only one pair of PCR primers is used. MLPA reactions with currently commercial available
kits result in very reproducible gel patterns with fragments of 130 to 480 bp that can be
analyzed by sequence type electrophoresis. Comparison of this gel pattern to that obtained
from a control sample indicates which sequences show an aberrant copy number.
Original languageEnglish
Title of host publicationPrenatal diagnosis
EditorsSinuhe Hahn, Laird G. Jackson
PublisherHumana Press
Number of pages12
ISBN (Electronic)978-1-59745-066-9
ISBN (Print)9781588298034
Publication statusPublished - 2008

Publication series

SeriesMethods in Molecular Biology

Bibliographical note

© Humana Press, Totowa, NJ

Research programs

  • EMC MGC-02-96-01


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