Abstract
Multiplex ligation-dependent probe amplification (MLPA) is a new method to
determine the copy number of up to 45 genomic DNA sequences in a single multiplex
polymerase chain reaction (PCR)-based reaction. In contrast to standard multiplex PCR,
only one pair of PCR primers is used. MLPA reactions with currently commercial available
kits result in very reproducible gel patterns with fragments of 130 to 480 bp that can be
analyzed by sequence type electrophoresis. Comparison of this gel pattern to that obtained
from a control sample indicates which sequences show an aberrant copy number.
determine the copy number of up to 45 genomic DNA sequences in a single multiplex
polymerase chain reaction (PCR)-based reaction. In contrast to standard multiplex PCR,
only one pair of PCR primers is used. MLPA reactions with currently commercial available
kits result in very reproducible gel patterns with fragments of 130 to 480 bp that can be
analyzed by sequence type electrophoresis. Comparison of this gel pattern to that obtained
from a control sample indicates which sequences show an aberrant copy number.
| Original language | English |
|---|---|
| Title of host publication | Prenatal diagnosis |
| Editors | Sinuhe Hahn, Laird G. Jackson |
| Publisher | Humana Press |
| Chapter | 8 |
| Pages | 111-122 |
| Number of pages | 12 |
| ISBN (Electronic) | 978-1-59745-066-9 |
| ISBN (Print) | 9781588298034 |
| DOIs | |
| Publication status | Published - 2008 |
Publication series
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 444 |
| ISSN | 1064-3745 |
Bibliographical note
© Humana Press, Totowa, NJResearch programs
- EMC MGC-02-96-01