Modeling cartilage pathology in mucopolysaccharidosis VI using iPSCs reveals early dysregulation of chondrogenic and metabolic gene expression

M. Broeders, Jgj van Rooij, E. Oussoren, Tjm van Gestel, Ca Smith, Sj Kimber, Rm Verdijk, Maem Wagenmakers, Jmp van den Hout, At van der Ploeg, R. Narcisi, Wwmp Pijnappel*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Mucopolysaccharidosis type VI (MPS VI) is a metabolic disorder caused by disease-associated variants in the Arylsulfatase B (ARSB) gene, resulting in ARSB enzyme deficiency, lysosomal glycosaminoglycan accumulation, and cartilage and bone pathology. The molecular response to MPS VI that results in cartilage pathology in human patients is largely unknown. Here, we generated a disease model to study the early stages of cartilage pathology in MPS VI. We generated iPSCs from four patients and isogenic controls by inserting the ARSB cDNA in the AAVS1 safe harbor locus using CRISPR/Cas9. Using an optimized chondrogenic differentiation protocol, we found Periodic acid–Schiff positive inclusions in hiPSC-derived chondrogenic cells with MPS VI. Genome-wide mRNA expression analysis showed that hiPSC-derived chondrogenic cells with MPS VI downregulated expression of genes involved in TGF-β/BMP signalling, and upregulated expression of inhibitors of the Wnt/β-catenin signalling pathway. Expression of genes involved in apoptosis and growth was upregulated, while expression of genes involved in glycosaminoglycan metabolism was dysregulated in hiPSC-derived chondrogenic cells with MPS VI. These results suggest that human ARSB deficiency in MPS VI causes changes in the transcriptional program underlying the early stages of chondrogenic differentiation and metabolism.

Original languageEnglish
Article number949063
JournalFrontiers in Bioengineering and Biotechnology
Volume10
DOIs
Publication statusPublished - 6 Dec 2022

Bibliographical note

Funding Information:
This work has received funding from Zeldzame Ziekten Fonds/WE Foundation, Metakids (project number 2018-082), and Stofwisselkracht. RN is part of the Erasmus Postgraduate School Molecular Medicine and Medical Delta Regenerative Medicine 4D program.

Publisher Copyright:
Copyright © 2022 Broeders, van Rooij, Oussoren, van Gestel, Smith, Kimber, Verdijk, Wagenmakers, van den Hout, van der Ploeg, Narcisi and Pijnappel.

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