Molecular characterization of piezotolerant and stress-resistant mutants of Staphylococcus aureus

K. A.G. Karatzas*, N. A. Lemmens-den Toom, C. C. Tassou, W. van Leeuwen, A. van Belkum

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
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Abstract

Aims: In the previous work, following a pressure treatment with wild-type Staphylococcus aureus, we obtained piezotolerant isolates showing altered phenotypic characteristics. This work focuses on understanding the genetic background of their altered phenotype. Methods and Results: AK23, a representative piezotolerant isolate was subjected to DNA microarrays, corroborated by PCR product sequencing and revealed 10-gene deletion. All other piezotolerant isolates possessed the mutation encompassing the region from SAR0665 to SAR0674 genes (9351 bp) which was most likely the result of recombination between two homologous loci (ATTGCGGGTG) present in both genes. RNA microarray transcriptomic analysis showed that due to partial deletion of the low-affinity phosphate transporter pitA, the high-affinity PhoU-PstABCS operon was upregulated in AK23 which could be the reason for piezotolerance. Furthermore, AK23 showed low levels of the virulence gene regulator rnaIII resulting in the downregulation of several agr system genes explaining the impaired virulence characteristics of the mutant. Conclusions: Naturally occurring mutations can result in piezotolerance which can be of a concern for high hydrostatic pressure-treated foods. Significance and Impact of the Study: A locus has been identified in piezotolerant S. aureus mutants providing insight into possible mechanisms associated with phenotypic characteristics of S. aureus. Further work should study each individual gene of the locus.

Original languageEnglish
Pages (from-to)901-912
Number of pages12
JournalJournal of Applied Microbiology
Volume130
Issue number3
Early online date1 Sept 2020
DOIs
Publication statusPublished - Mar 2021

Bibliographical note

Funding Information:
We would like to thank all colleagues at the Microbiology research team, Food and Nutritional Sciences, University of Reading. The help and support of our deceased colleague Dr. Bernard Mackey has been invaluable. This work was partly supported by a Marie Curie European Reintegration Grant (grant ERG 265154) awarded to K.A.G. Karatzas, and funds from the University of Reading.

Publisher Copyright:
© 2020 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology

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