MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION: Effects of oltipraz, α-tocopherol, β-carotene and phenethylisothiocyanate on rat oesophageal, gastric, colonic and hepatic glutathione, glutathione S-transferase and peroxidase

Esther M.M. Van Lieshout, Wilbert H.M. Peters*, Jan B.M.J. Jansen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Four anticarcinogens (oltipraz, α-tocopherol, β-carotene and phenethylisothiocyanate [PEITC]) were studied with respect to their effects on oesophageal, gastric, colonic and hepatic (i) glutathione (GSH) content, (ii) glutathione S-transferase (GST) enzyme activity, (iii) GST isoenzyme levels, and (iv) glutathione peroxidase (GPx) enzyme activity in male Wistar rats. GST enzyme activity was significantly increased in oesophagus (1.9 ×) and colon (1.2×) by PEITC and in liver (1.4×) by oltipraz. GST Alpha was doubled in the liver by oltipraz, α-tocopherol and PEITC. GST Mu levels were increased by β-carotene and PEITC in stomach and liver, by oltipraz in liver and by α-tocopherol in stomach. PEITC induced colonic GST Pi levels (13×). GSH content was induced in liver by oltipraz (1.4×) and α-tocopherol (1.2×) and in colon by PEITC (1.6×). Each of the anticarcinogens tested increased GPx activity at one or more sites: Se-dependent and total GPx activities were induced in 31.3% and 37.5% of all possibilities, respectively. Major induction in total GPx was found in stomach by α-tocopherol (1.8×). In conclusion our data demonstrate that dietary administration of oltipraz, PEITC, α-tocopherol and β-carotene, may exert chemopreventive effects in the digestive tract of the rat by enhancing GST, GPx, and, to a lesser extent, GSH levels.
Original languageEnglish
Pages (from-to)1439-1445
JournalCarcinogenesis
Volume17
Issue number7
DOIs
Publication statusPublished - Jul 1996
Externally publishedYes

Bibliographical note

Acknowledgements
The authors would like to thank Hennie M.J.Roelofs and Dr Wim A.Nijhoff for their excellent technical assistance. This work was supported by grant
KUN 94-715 (EMMvL) from the Dutch Cancer Society.

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