Molecular recognition of DNA-protein complexes: A straightforward method combining scanning force and fluorescence microscopy

H Sanchez; Roland Kanaar; C.L. Wyman

doi:10.1016/j.ultramic.2010.03.002

Molecular recognition of DNA-protein complexes: A straightforward method combining scanning force and fluorescence microscopy

H Sanchez, Roland Kanaar, C.L. Wyman

Research output: Contribution to journal › Article › Academic › peer-review

20 Citations (Scopus)

Abstract

Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility of this method for analysis of proteins and protein-DNA complexes. (C) 2010 Elsevier B.V. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	844-851
Number of pages	8
Journal	Ultramicroscopy
Volume	110
Issue number	7
DOIs	https://doi.org/10.1016/j.ultramic.2010.03.002
Publication status	Published - 2010

Research programs

EMC MGC-01-12-03
EMC MM-03-32-04

Access to Document

10.1016/j.ultramic.2010.03.002

http://hdl.handle.net/1765/27953

Cite this

@article{fdf25b7753a44ee2b39699d7a714df36,

title = "Molecular recognition of DNA-protein complexes: A straightforward method combining scanning force and fluorescence microscopy",

abstract = "Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility of this method for analysis of proteins and protein-DNA complexes. (C) 2010 Elsevier B.V. All rights reserved.",

author = "H Sanchez and Roland Kanaar and C.L. Wyman",

year = "2010",

doi = "10.1016/j.ultramic.2010.03.002",

language = "Undefined/Unknown",

volume = "110",

pages = "844--851",

journal = "Ultramicroscopy",

issn = "0304-3991",

publisher = "Elsevier",

number = "7",

}

TY - JOUR

T1 - Molecular recognition of DNA-protein complexes: A straightforward method combining scanning force and fluorescence microscopy

AU - Sanchez, H

AU - Kanaar, Roland

AU - Wyman, C.L.

PY - 2010

Y1 - 2010

N2 - Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility of this method for analysis of proteins and protein-DNA complexes. (C) 2010 Elsevier B.V. All rights reserved.

AB - Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility of this method for analysis of proteins and protein-DNA complexes. (C) 2010 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.ultramic.2010.03.002

DO - 10.1016/j.ultramic.2010.03.002

M3 - Article

C2 - 20382478

SN - 0304-3991

VL - 110

SP - 844

EP - 851

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 7

ER -