TY - JOUR
T1 - Monitoring Immune Cell Function Through Optical Imaging
T2 - a Review Highlighting Transgenic Mouse Models
AU - Chawda, Chintan
AU - McMorrow, Roisin
AU - Gaspar, Natasa
AU - Zambito, Giorgia
AU - Mezzanotte, Laura
N1 - Publisher Copyright: © 2021, The Author(s).
PY - 2022/4
Y1 - 2022/4
N2 - Transgenic mouse models have facilitated research of human diseases and validation of therapeutic approaches. Inclusion of optical reporter genes (fluorescent or bioluminescent genes) in the targeting vectors used to develop such models makes in vivo imaging of cellular and molecular events possible, from the microscale to the macroscale. In particular, transgenic mouse models expressing optical reporter genes allowed accurately distinguishing immune cell types from trafficking in vivo using intravital microscopy or whole-body optical imaging. Besides lineage tracing and trafficking of different subsets of immune cells, the ability to monitor the function of immune cells is of pivotal importance for investigating the effects of immunotherapies against cancer. Here, we introduce the reader to state-of-the-art approaches to develop transgenics, optical imaging techniques, and several notable examples of transgenic mouse models developed for immunology research by critically highlighting the models that allow the following of immune cell function.
AB - Transgenic mouse models have facilitated research of human diseases and validation of therapeutic approaches. Inclusion of optical reporter genes (fluorescent or bioluminescent genes) in the targeting vectors used to develop such models makes in vivo imaging of cellular and molecular events possible, from the microscale to the macroscale. In particular, transgenic mouse models expressing optical reporter genes allowed accurately distinguishing immune cell types from trafficking in vivo using intravital microscopy or whole-body optical imaging. Besides lineage tracing and trafficking of different subsets of immune cells, the ability to monitor the function of immune cells is of pivotal importance for investigating the effects of immunotherapies against cancer. Here, we introduce the reader to state-of-the-art approaches to develop transgenics, optical imaging techniques, and several notable examples of transgenic mouse models developed for immunology research by critically highlighting the models that allow the following of immune cell function.
UR - http://www.scopus.com/inward/record.url?scp=85118588173&partnerID=8YFLogxK
U2 - 10.1007/s11307-021-01662-5
DO - 10.1007/s11307-021-01662-5
M3 - Review article
C2 - 34735680
AN - SCOPUS:85118588173
SN - 1536-1632
VL - 24
SP - 250
EP - 263
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
IS - 2
ER -