Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H 16) with Raman spectroscopy

Jacoline Gelder, D Willemse-Erix, MJ Scholtes, JI Sanchez, Kees Maquelin, P Vandenabeele, P De Boever, Gerwin Puppels, L Moens, Patricia Vos

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Abstract

This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.
Original languageUndefined/Unknown
Pages (from-to)2155-2160
Number of pages6
JournalAnalytical Chemistry
Volume80
Issue number6
DOIs
Publication statusPublished - 2008

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