Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H 16) with Raman spectroscopy

Jacoline Gelder; D Willemse-Erix; MJ Scholtes; JI Sanchez; Kees Maquelin; P Vandenabeele; P De Boever; Gerwin Puppels; L Moens; Patricia Vos

doi:10.1021/ac702185d

Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H 16) with Raman spectroscopy

Jacoline Gelder, D Willemse-Erix, MJ Scholtes, JI Sanchez, Kees Maquelin, P Vandenabeele, P De Boever, Gerwin Puppels, L Moens, Patricia Vos

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.

Original language	Undefined/Unknown
Pages (from-to)	2155-2160
Number of pages	6
Journal	Analytical Chemistry
Volume	80
Issue number	6
DOIs	https://doi.org/10.1021/ac702185d
Publication status	Published - 2008

Research programs

EMC MM-03-61-05-A

Access to Document

10.1021/ac702185d

http://hdl.handle.net/1765/32395

Cite this

@article{726defe138124e76bf39541caca402a9,

title = "Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H 16) with Raman spectroscopy",

abstract = "This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.",

author = "Jacoline Gelder and D Willemse-Erix and MJ Scholtes and JI Sanchez and Kees Maquelin and P Vandenabeele and \{De Boever\}, P and Gerwin Puppels and L Moens and Patricia Vos",

year = "2008",

doi = "10.1021/ac702185d",

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journal = "Analytical Chemistry",

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TY - JOUR

T1 - Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H 16) with Raman spectroscopy

AU - Gelder, Jacoline

AU - Willemse-Erix, D

AU - Scholtes, MJ

AU - Sanchez, JI

AU - Maquelin, Kees

AU - Vandenabeele, P

AU - De Boever, P

AU - Puppels, Gerwin

AU - Moens, L

AU - Vos, Patricia

PY - 2008

Y1 - 2008

N2 - This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.

AB - This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.

U2 - 10.1021/ac702185d

DO - 10.1021/ac702185d

M3 - Article

C2 - 18266340

SN - 0003-2700

VL - 80

SP - 2155

EP - 2160

JO - Analytical Chemistry

JF - Analytical Chemistry

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