Previously reported short-term results after live kidney donation show no negative consequences for the donor. The incidence of new-onset morbidity takes years to emerge, making it highly likely that this will be missed during short-term follow-up. Therefore, evidence on long-term outcome is essential. A 10-year follow-up on renal function, hypertension, quality of life (QOL), fatigue, and survival was performed of a prospective cohort of 100 donors. After a median follow-up time of 10years, clinical data were available for 97 donors and QOL data for 74 donors. Nine donors died during follow-up of unrelated causes to donation, and one donor was lost to follow-up. There was a significant decrease in kidney function of 12.9ml/min (P<0.001) at follow-up. QOL showed significant clinically relevant decreases of 10-year follow-up scores in SF-36 dimensions of physical function (P<0.001), bodily pain (P=0.001), and general health (P<0.001). MFI-20 scores were significantly higher for general fatigue (P<0.001), physical fatigue (P<0.001), reduced activity (P=0.019), and reduced motivation (P=0.030). New-onset hypertension was present in 25.6% of the donors. Donor outcomes are excellent 10years post-donation. Kidney function appears stable, and hypertension does not seem to occur more frequently compared to the general population.