Mucosal pentraxin (Mptx), a novel rat gene ten-fold downregulated in colon by dietary heme

Cindy Van Der Meer-Van Kraaij, Esther van Lieshout, Evelien Kramer, Roelof Van Der Meer, Jaap Keijer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)
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Abstract

Consumption of red meat is associated with increased colon cancer risk. Our previous work indicated that this association might be due to the heme content of red meat. In rat studies, dietary heme increased colonic cytotoxicity and epithelial cell turnover, carcinogenesis biomarkers. Here we apply DNA microarray technology to examine effects of heme on colonic gene expression. A rat colon-specific microarray was constructed and hybridized in duplicate to RNA extracts from colon scrapings of rats fed diets with or without heme (n=6–7). We were able to reproducibly identify changes in colonic mRNA abundance in response to heme. Most striking was a >10-fold down-regulation of a single rat gene, an unprecedented gene-modulating effect of a dietary component. Based on homology, the novel gene encodes a pentraxin, the first identified in colon. Pentraxins are postulated to be involved in dealing with dying cells. Quantitative PCR confirmed the strong heme-induced down-regulation of this gene, which we named mucosal pentraxin (Mptx). Overall, our data support the efficacy of cDNA array expression profiling to investigate effects of specific nutrients in an in vivo system and may provide an approach to establishing markers for diet-induced stress of mammalian colonic mucosa.—Ovan der Meervan Kraaij, C., van Lieshout, E. M. M., Kramer, E., van der Meer, R., Keijer, J. Mucosal pentraxin (Mptx), a novel rat gene 10-fold down-regulated in colon by dietary heme. FASEB J. 17, 1277–1285 (2003)
Original languageEnglish
Pages (from-to)1277-1286
JournalFASEB Journal
Volume17
Issue number10
DOIs
Publication statusPublished - Jul 2003
Externally publishedYes

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