Multi-modal image registration: Matching MRI with histology

Lejla Alic*, Joost C. Haeck, Stefan Klein, Karin Bol, Sandra T. Van Tiel, Piotr A. Wielopolski, Magda Bijster, Wiro J. Niessen, Monique Bernsen, Jifke F. Veenland, Marion De Jong

*Corresponding author for this work

Research output: Chapter/Conference proceedingConference proceedingAcademicpeer-review

8 Citations (Scopus)


Spatial correspondence between histology and multi sequence MRI can provide information about the capabilities of non-invasive imaging to characterize cancerous tissue. However, shrinkage and deformation occurring during the excision of the tumor and the histological processing complicate the co registration of MR images with histological sections. This work proposes a methodology to establish a detailed 3D relation between histology sections and in vivo MRI tumor data. The key features of the methodology are a very dense histological sampling (up to 100 histology slices per tumor), mutual information based non-rigid B-spline registration, the utilization of the whole 3D data sets, and the exploitation of an intermediate ex vivo MRI. In this proof of concept paper, the methodology was applied to one tumor. We found that, after registration, the visual alignment of tumor borders and internal structures was fairly accurate. Utilizing the intermediate ex vivo MRI, it was possible to account for changes caused by the excision of the tumor: we observed a tumor expansion of 20%. Also the effects of fixation, dehydration and histological sectioning could be determined: 26% shrinkage of the tumor was found. The annotation of viable tissue, performed in histology and transformed to the in vivo MRI, matched clearly with high intensity regions in MRI. With this methodology, histological annotation can be directly related to the corresponding in vivo MRI. This is a vital step for the evaluation of the feasibility of multi-spectral MRI to depict histological ground-truth.

Original languageEnglish
Title of host publicationMedical Imaging 2010 - Biomedical Applications in Molecular, Structural, and Functional Imaging
Publication statusPublished - 9 Mar 2010
EventMedical Imaging 2010 - Biomedical Applications in Molecular, Structural, and Functional Imaging - San Diego, CA, United States
Duration: 14 Feb 201016 Feb 2010

Publication series

SeriesProgress in Biomedical Optics and Imaging - Proceedings of SPIE


ConferenceMedical Imaging 2010 - Biomedical Applications in Molecular, Structural, and Functional Imaging
Country/TerritoryUnited States
CitySan Diego, CA


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