Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker

Dea Garcia-Hermoso; Donna M MacCallum; Timothy J Lott; Paula Sampaio; Maria-José Buitrago Serna; Fréderic Grenouillet; Corné H W Klaassen; Stéphane Bretagne

doi:10.1128/JCM.00040-10

Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker

Dea Garcia-Hermoso, Donna M MacCallum, Timothy J Lott, Paula Sampaio, Maria-José Buitrago Serna, Fréderic Grenouillet, Corné H W Klaassen, Stéphane Bretagne

Research output: Contribution to journal › Article › Academic › peer-review

16 Citations (Scopus)

Abstract

Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.

Original language	English
Pages (from-to)	2578-81
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	48
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00040-10
Publication status	Published - Jul 2010
Externally published	Yes

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title = "Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker",

abstract = "Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.",

author = "Dea Garcia-Hermoso and MacCallum, {Donna M} and Lott, {Timothy J} and Paula Sampaio and Serna, {Maria-Jos{\'e} Buitrago} and Fr{\'e}deric Grenouillet and Klaassen, {Corn{\'e} H W} and St{\'e}phane Bretagne",

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language = "English",

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AU - Garcia-Hermoso, Dea

AU - MacCallum, Donna M

AU - Lott, Timothy J

AU - Sampaio, Paula

AU - Serna, Maria-José Buitrago

AU - Grenouillet, Fréderic

AU - Klaassen, Corné H W

AU - Bretagne, Stéphane

PY - 2010/7

Y1 - 2010/7

N2 - Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.

AB - Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.

U2 - 10.1128/JCM.00040-10

DO - 10.1128/JCM.00040-10

M3 - Article

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SN - 0095-1137

VL - 48

SP - 2578

EP - 2581

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker

Abstract

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