Multilocus Genetic Risk Score Associates With Ischemic Stroke in Case-Control and Prospective Cohort Studies

R Malik, S Bevan, MA Nalls, EG Holliday, WJ Devan, YC Cheng, Carla Verbaas, Ben Verhaaren, JC Bis, AY (Aaron) Joon, AL de Stefano, M Fornage, BM Psaty, Arfan Ikram, LJ (Lenore) Launer, Cornelia Duijn, P Sharma, BD Mitchell, J Rosand, JF MeschiaC Levi, PM Rothwell, C Sudlow, HS Markus, S Seshadri, M Dichgans

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Abstract

Background and Purpose Genome-wide association studies have revealed multiple common variants associated with known risk factors for ischemic stroke (IS). However, their aggregate effect on risk is uncertain. We aimed to generate a multilocus genetic risk score (GRS) for IS based on genome-wide association studies data from clinical-based samples and to establish its external validity in prospective population-based cohorts. Methods Three thousand five hundred forty-eight clinic-based IS cases and 6399 controls from the Wellcome Trust Case Control Consortium 2 were used for derivation of the GRS. Subjects from the METASTROKE consortium served as a replication sample. The validation sample consisted of 22 751 participants from the Cohorts for Heart and Aging Research in Genomic Epidemiology consortium. We selected variants that had reached genome-wide significance in previous association studies on established risk factors for IS. Results A combined GRS for atrial fibrillation, coronary artery disease, hypertension, and systolic blood pressure significantly associated with IS both in the case-control samples and in the prospective population-based studies. Subjects in the top quintile of the combined GRS had >2-fold increased risk of IS compared with subjects in the lowest quintile. Addition of the combined GRS to a simple model based on sex significantly improved the prediction of IS in the combined clinic-based samples but not in the population-based studies, and there was no significant improvement in net reclassification. Conclusions A multilocus GRS based on common variants for established cardiovascular risk factors was significantly associated with IS both in clinic-based samples and in the general population. However, the improvement in clinical risk prediction was found to be small.
Original languageUndefined/Unknown
Pages (from-to)394-402
Number of pages9
JournalStroke
Volume45
Issue number2
DOIs
Publication statusPublished - 2014

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