Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids

Hwidon Lee; Markus R. Seeger; Norman Lippok; Seemantini K. Nadkarni; Gijs van Soest; Brett E. Bouma

doi:10.1016/j.pacs.2022.100331

Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids

Hwidon Lee, Markus R. Seeger, Norman Lippok, Seemantini K. Nadkarni, Gijs van Soest, Brett E. Bouma^*

^*Corresponding author for this work

Erasmus MC other

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Near-infrared photoacoustics receives increasing interest as an intravital modality to sense key biomolecules. One of the most central types of biomolecules of interest are lipids as they constitute essential bio-hallmarks of cardiovascular and metabolic diseases and their in-vivo detection holds insightful information about disease progression and treatment monitoring. However, the full potential of near-infrared photoacoustic for high-resolution and high-sensitivity biomedical studies of lipids has so far not been exploited due a lack of appropriate excitation sources delivering short-pulses at high-repetition-rate, high-pulse-energy, and wavelength around 1200 nm. Here, we demonstrate a custom-built SRS fiber amplifier that provides optical excitations at 1192.8 nm, repetition rates of 200 kHz, pulse durations below 2 ns, and pulse energies beyond 5 μJ. We capitalize on the performance of our excitation source and show near-infrared photoacoustics resolving intrinsic lipid contrast in biomedically relevant specimens ranging from single cells to lipid-rich tissue with subcellular resolution.

Original language	English
Article number	100331
Journal	Photoacoustics
Volume	25
Early online date	15 Jan 2022
DOIs	https://doi.org/10.1016/j.pacs.2022.100331
Publication status	Published - 20 Jan 2022

Bibliographical note

Funding Information:
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Brett E. Bouma reports financial support was provided by National Institutes of Health. Markus R. Seeger reports financial support was provided by The Optical Society. Gijs van Soest reports a relationship with Kaminari Medical BV that includes: equity or stocks. Seemantini K. Nadkarni reports a relationship with Coalesenz Inc. that includes: equity or stocks.

Funding Information:
This work was supported by the National Institutes of Health , USA ( P41 EB015903 ). MRS was supported by the OSA Thomas F. Deutsch Fellowship, Optica (formerly known as The Optical Society), USA.

Publisher Copyright:
© 2022

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title = "Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids",

abstract = "Near-infrared photoacoustics receives increasing interest as an intravital modality to sense key biomolecules. One of the most central types of biomolecules of interest are lipids as they constitute essential bio-hallmarks of cardiovascular and metabolic diseases and their in-vivo detection holds insightful information about disease progression and treatment monitoring. However, the full potential of near-infrared photoacoustic for high-resolution and high-sensitivity biomedical studies of lipids has so far not been exploited due a lack of appropriate excitation sources delivering short-pulses at high-repetition-rate, high-pulse-energy, and wavelength around 1200 nm. Here, we demonstrate a custom-built SRS fiber amplifier that provides optical excitations at 1192.8 nm, repetition rates of 200 kHz, pulse durations below 2 ns, and pulse energies beyond 5 μJ. We capitalize on the performance of our excitation source and show near-infrared photoacoustics resolving intrinsic lipid contrast in biomedically relevant specimens ranging from single cells to lipid-rich tissue with subcellular resolution.",

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Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids

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