Near-infrared bioluminescence imaging of two cell populations in living mice

Giorgia Zambito, Laura Mezzanotte*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Multicolor bioluminescence imaging using near-infrared emitting luciferases is an attractive application to detect two cell populations within one animal model. Herein, we describe how to distinguish dual-color bioluminescent signals co-localized in the same compartment. We tested CBG2 click beetle (λ = 660 nm) and CBR2 click beetle (λ = 730 nm) luciferases paired with NH2-NpLH2 luciferin. Following a spectral unmixing algorithm, single spectral contributions can be resolved and quantified, enabling the visualization of multiple cell types in deep tissue by injection of a single substrate. For complete details on the use and execution of this protocol, please refer to Zambito et al. (2020).

Original languageEnglish
Article number100662
JournalSTAR Protocols
Volume2
Issue number3
DOIs
Publication statusPublished - 17 Sep 2021

Bibliographical note

Funding Information:
We acknowledge the funding for this work provided by the European Commission under the H2020 -MSCA-RISE award grant number 777682 (CANCER). The graphical abstract and figures were partially created with BioRender.com .

Publisher Copyright:
© 2021 The Author(s)

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