Non-HLA T-cell reactivity during the first year after HLA-identical living-related kidney transplantation

JH (Jeroen) Gerrits, Jacqueline van de Wetering, JJ Drabbels, J.N.M. IJzermans, FHJ Claas, Willem Weimar, Nicole van Besouw

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Abstract

Background: It has been reported that donor-reactive T-cell responses may decrease during the first year after HLA-mismatched organ transplantation. We wondered whether donor-reactive T-cell responses directed to minor histocompatibility antigens (mHAgs) or other non-HLA antigens also decrease after HLA-identical living-related (LR) kidney transplantation. Methods: We studied donor-reactive T-cell responses by IFN-gamma and granzyme B (GrB) Elispot assays in 15 HLA-identical LR kidney transplant recipients before, six months and one yr after transplantation. Third-party reactivity was used as control. Patient and donor peripheral blood mononuclear cells were typed for 11 known mHAgs. Results: During the study period, 60% and 36% of the patients demonstrated donor-reactive IFN-gamma and GrB producing cells (pc), respectively. The number of donor-reactive IFN-gamma and GrB pc was significantly lower than the number of third-party reactive IFN-gamma and GrB pc. After transplantation, donor-reactivity and third-party reactivity were comparable to pre-transplant values. No relation was found in mHAg mismatches between donor and recipient and donor-reactive T-cell response. Conclusions: Donor-reactivity could be detected before and after HLA-identical LR kidney transplantation, but was not related with the number of mHAg mismatches, and did not decrease after transplantation.
Original languageUndefined/Unknown
Pages (from-to)740-747
Number of pages8
JournalClinical Transplantation
Volume23
Issue number5
DOIs
Publication statusPublished - 2009

Research programs

  • EMC MM-04-39-05
  • EMC MM-04-47-07

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