Off-resonance saturation as an MRI method to quantify mineral- iron in the post-mortem brain

Lucia Bossoni*, Ingrid Hegeman-Kleinn, Sjoerd G. van Duinen, Marjolein Bulk, Lena H.P. Vroegindeweij, Janneke G. Langendonk, Lydiane Hirschler, Andrew Webb, Louise van der Weerd

*Corresponding author for this work

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Purpose: To employ an off-resonance saturation method to measure the mineral-iron pool in the postmortem brain, which is an endogenous contrast agent that can give information on cellular iron status. Methods: An off-resonance saturation acquisition protocol was implemented on a 7 Tesla preclinical scanner, and the contrast maps were fitted to an established analytical model. The method was validated by correlation and Bland-Altman analysis on a ferritin-containing phantom. Mineral-iron maps were obtained from postmortem tissue of patients with neurological diseases characterized by brain iron accumulation, that is, Alzheimer disease, Huntington disease, and aceruloplasminemia, and validated with histology. Transverse relaxation rate and magnetic susceptibility values were used for comparison. Results: In postmortem tissue, the mineral-iron contrast colocalizes with histological iron staining in all the cases. Iron concentrations obtained via the off-resonance saturation method are in agreement with literature. Conclusions: Off-resonance saturation is an effective way to detect iron in gray matter structures and partially mitigate for the presence of myelin. If a reference region with little iron is available in the tissue, the method can produce quantitative iron maps. This method is applicable in the study of diseases characterized by brain iron accumulation and can complement existing iron-sensitive parametric methods.

Original languageEnglish
Pages (from-to)1276-1288
Number of pages13
JournalMagnetic Resonance in Medicine
Issue number3
Early online date15 Oct 2021
Publication statusPublished - Mar 2022

Bibliographical note

Funding Information:
This study was supported by the Netherlands Organization for Scientific Research (NWO) through a VENI fellowship to l.b. (0.16.Veni.188.040)

Publisher Copyright:
© 2021 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.


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