Optical imaging of oral squamous cell carcinoma and cervical lymph node metastasis

Stijn Keereweer, JDF Kerrebijn, IM Mol, JSD Mieog, Kishan Tsang, R.J. Baatenburg de Jong, AL Vahrmeijer, Clemens Löwik

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38 Citations (Scopus)

Abstract

Background In oral cancer surgery, intraoperative optical imaging could help the surgeon to determine adequate tumor-free margins. Methods Tumor-specific near-infrared fluorescence agents targeting epidermal growth factor receptor (CW800 EGF) or glucose transporter system (CW800 2-DG) were administered to mice with tongue carcinoma and cervical lymph node metastases. Tumor growth was followed by bioluminescence imaging. Fluorescence signals were compared with a control group of healthy animals. Results Significantly higher fluorescence was found in tongue tumors and cervical lymph node metastases compared with that in control animals. Fluorescence correlated with histopathology. Tumor-to-background ratio of CW800 EGF in the tongue was 13.8 (SD = 6.1) and in the lymph nodes 15.7 (SD = 8.8). For CW800 2-DG, the tumor-to-background ratio in the tongue was 4.6 (SD = 2.1) and in the lymph nodes 33.9 (SD = 18.4). Conclusions Optical imaging can be used to detect oral cancer and cervical lymph node metastases and could potentially improve complete surgical resection by real-time image-guided surgery. (c) 2011 Wiley Periodicals, Inc. Head Neck, 2012
Original languageUndefined/Unknown
Pages (from-to)1002-1008
Number of pages7
JournalHead & Neck-Journal for the Sciences and Specialties of the Head and Neck
Volume34
Issue number7
DOIs
Publication statusPublished - 2012

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