@inbook{3359560b5c624fad8d9119ea861e95de,
title = "Optimal Sample Preparation Workflow for Quantitative Mass Spectrometry-Based Studies in the Low Range",
abstract = "Mass spectrometry (MS) enables the high-throughput characterization of thousands of proteins in one single run. Nowadays, advances in the field have allowed for deep analysis of low cell numbers, even reaching the single-cell level. However, standardized protocols are still needed in this regard, especially oriented for non-proteomics specialists. Here we detail a reproducible, easy-to-implement workflow for cell-limited sample preparation, from cell storage to peptide labelling, for further TMT-based relative quantitative MS-based analysis.",
author = "Sara Kassem and Paula D{\'i}ez",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",
year = "2025",
doi = "10.1007/978-1-0716-4298-6\_2",
language = "English",
isbn = "978-1-0716-4297-9",
series = "Methods in Molecular Biology",
pages = "13--23",
editor = "\{Islam Williams\}, Taufika",
booktitle = "Methods in Molecular Biology",
}