Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts

Laura C. Burlage*, Alexandre G. Lellouch, Corentin B. Taveau, Philipp Tratnig-Frankl, Casie A. Pendexter, Mark A. Randolph, Robert J. Porte, Laurent A. Lantieri, Shannon N. Tessier, Curtis L. Cetrulo, Korkut Uygun

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Background: 

Machine perfusion is gaining interest as an efficient method of tissue preservation of Vascularized Composite Allografts (VCA). The aim of this study was to develop a protocol for ex vivo subnormothermic oxygenated machine perfusion (SNMP) on rodent hindlimbs and to validate our protocol in a heterotopic hindlimb transplant model. 

Methods: 

In this optimization study we compared three different solutions during 6 h of SNMP (n = 4 per group). Ten control limbs were stored in a preservation solution on Static Cold Storage [SCS]). During SNMP we monitored arterial flowrate, lactate levels, and edema. After SNMP, muscle biopsies were taken for histology examination, and energy charge analysis. We validated the best perfusion protocol in a heterotopic limb transplantation model with 30-d follow up (n = 13). As controls, we transplanted untreated limbs (n = 5) and hindlimbs preserved with either 6 or 24 h of SCS (n = 4 and n = 5). 

Results: 

During SNMP, arterial outflow increased, and lactate clearance decreased in all groups. Total edema was significantly lower in the HBOC-201 group compared to the BSA group (P = 0.005), 4.9 (4.3-6.1) versus 48.8 (39.1-53.2) percentage, but not to the BSA + PEG group (P = 0.19). Energy charge levels of SCS controls decreased 4-fold compared to limbs perfused with acellular oxygen carrier HBOC-201, 0.10 (0.07-0.17) versus 0.46 (0.42-0.49) respectively (P = 0.002). 

Conclusions: 

Six hours ex vivo SNMP of rodent hindlimbs using an acellular oxygen carrier HBOC-201 results in superior tissue preservation compared to conventional SCS.

Original languageEnglish
Pages (from-to)151-161
Number of pages11
JournalJournal of Surgical Research
Volume270
DOIs
Publication statusPublished - Feb 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021

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