TY - JOUR
T1 - Oraldisk
T2 - A chair-side compatible molecular platform using whole saliva for monitoring oral health at the dental practice
AU - Baumgartner, Desirée
AU - Johannsen, Benita
AU - Specht, Mara
AU - Lüddecke, Jan
AU - Rombach, Markus
AU - Hin, Sebastian
AU - Paust, Nils
AU - von Stetten, Felix
AU - Zengerle, Roland
AU - Herz, Christopher
AU - Peham, Johannes R.
AU - Paqué, Pune N.
AU - Attin, Thomas
AU - Jenzer, Joël S.
AU - Körner, Philipp
AU - Schmidlin, Patrick R.
AU - Thurnheer, Thomas
AU - Wegehaupt, Florian J.
AU - Kaman, Wendy E.
AU - Stubbs, Andrew
AU - Hays, John P.
AU - Rusu, Viorel
AU - Michie, Alex
AU - Binsl, Thomas
AU - Stejskal, David
AU - Karpíšek, Michal
AU - Bao, Kai
AU - Bostanci, Nagihan
AU - Belibasakis, Georgios N.
AU - Mitsakakis, Konstantinos
N1 - Funding Information:
Funding: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 633780 (‘DIAGORAS’ project), as well as the KI/SLL Styrgruppen för Odontologisk Forskning (SOF) Dnr. 4-823/2019. The article processing charge was funded by the Baden-Wuerttemberg Ministry of Science, Research and Art and the University of Freiburg in the funding programme Open Access Publishing.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients’ general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola) and three caries-associated (oral Lactobacilli, Streptococcus mutans, Streptococcus sobrinus). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk’s potential and suitability for inclusion in larger prospective implementation studies in dental care settings.
AB - Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients’ general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola) and three caries-associated (oral Lactobacilli, Streptococcus mutans, Streptococcus sobrinus). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk’s potential and suitability for inclusion in larger prospective implementation studies in dental care settings.
UR - http://www.scopus.com/inward/record.url?scp=85118535345&partnerID=8YFLogxK
U2 - 10.3390/bios11110423
DO - 10.3390/bios11110423
M3 - Article
AN - SCOPUS:85118535345
SN - 2079-6374
VL - 11
JO - Biosensors
JF - Biosensors
IS - 11
M1 - 423
ER -
