Oxygen-dependent delayed fluorescence measured in skin after topical application of 5-aminolevulinic acid

Floor Harms; WMI de Boon; Gianmarco Balestra; Sander Bodmer; Tanja Johannes; Robert jan Stolker; Bert Mik

doi:10.1002/jbio.201100040

Oxygen-dependent delayed fluorescence measured in skin after topical application of 5-aminolevulinic acid

Floor Harms, WMI de Boon, Gianmarco Balestra, Sander Bodmer, Tanja Johannes, Robert jan Stolker, Bert Mik

Anesthesiology

Research output: Contribution to journal › Article › Academic › peer-review

47 Citations (Scopus)

Abstract

Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than monoexponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique. [GRAPHICS] Oxygen-dependent delayed fluorescence measured on the forearm of a volunteer before and after inflation of a blood pressure cuff.

Original language	Undefined/Unknown
Pages (from-to)	731-739
Number of pages	9
Journal	Journal of Biophotonics
Volume	4
Issue number	10
DOIs	https://doi.org/10.1002/jbio.201100040
Publication status	Published - 2011

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10.1002/jbio.201100040

http://hdl.handle.net/1765/30736

Cite this

@article{6b34b0c9ca174a678a466de8bc60506f,

title = "Oxygen-dependent delayed fluorescence measured in skin after topical application of 5-aminolevulinic acid",

abstract = "Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than monoexponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique. [GRAPHICS] Oxygen-dependent delayed fluorescence measured on the forearm of a volunteer before and after inflation of a blood pressure cuff.",

author = "Floor Harms and {de Boon}, WMI and Gianmarco Balestra and Sander Bodmer and Tanja Johannes and Stolker, {Robert jan} and Bert Mik",

year = "2011",

doi = "10.1002/jbio.201100040",

language = "Undefined/Unknown",

volume = "4",

pages = "731--739",

journal = "Journal of Biophotonics",

issn = "1864-063X",

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T1 - Oxygen-dependent delayed fluorescence measured in skin after topical application of 5-aminolevulinic acid

AU - Harms, Floor

AU - de Boon, WMI

AU - Balestra, Gianmarco

AU - Bodmer, Sander

AU - Johannes, Tanja

AU - Stolker, Robert jan

AU - Mik, Bert

PY - 2011

Y1 - 2011

N2 - Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than monoexponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique. [GRAPHICS] Oxygen-dependent delayed fluorescence measured on the forearm of a volunteer before and after inflation of a blood pressure cuff.

AB - Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than monoexponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique. [GRAPHICS] Oxygen-dependent delayed fluorescence measured on the forearm of a volunteer before and after inflation of a blood pressure cuff.

U2 - 10.1002/jbio.201100040

DO - 10.1002/jbio.201100040

M3 - Article

C2 - 21770036

SN - 1864-063X

VL - 4

SP - 731

EP - 739

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 10

ER -