TY - JOUR
T1 - Oxygen transport during ex situ machine perfusion of donor livers using red blood cells or artificial oxygen carriers
AU - Bodewes, Silke B.
AU - van Leeuwen, Otto B.
AU - Thorne, Adam M.
AU - Lascaris, Bianca
AU - Ubbink, Rinse
AU - Lisman, Ton
AU - Monbaliu, Diethard
AU - De Meijer, Vincent E.
AU - Nijsten, Maarten W.N.
AU - Porte, Robert J.
N1 - Funding:
This research received no external funding.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Oxygenated ex situ machine perfusion of donor livers is an alternative for static cold preservation that can be performed at temperatures from 0◦C to 37◦C. Organ metabolism depends on oxygen to produce adenosine triphosphate and temperatures below 37◦C reduce the metabolic rate and oxygen requirements. The transport and delivery of oxygen in machine perfusion are key determinants in preserving organ viability and cellular function. Oxygen delivery is more challenging than carbon dioxide removal, and oxygenation of the perfusion fluid is temperature dependent. The maximal oxygen content of water-based solutions is inversely related to the temperature, while cellular oxygen demand correlates positively with temperature. Machine perfusion above 20◦C will therefore require an oxygen carrier to enable sufficient oxygen delivery to the liver. Human red blood cells are the most physiological oxygen carriers. Alternative artificial oxygen transporters are hemoglobin-based oxygen carriers, perfluorocarbons, and an extracellular oxygen carrier derived from a marine invertebrate. We describe the principles of oxygen transport, delivery, and consumption in machine perfusion for donor livers using different oxygen carrier-based perfusion solutions and we discuss the properties, advantages, and disadvantages of these carriers and their use.
AB - Oxygenated ex situ machine perfusion of donor livers is an alternative for static cold preservation that can be performed at temperatures from 0◦C to 37◦C. Organ metabolism depends on oxygen to produce adenosine triphosphate and temperatures below 37◦C reduce the metabolic rate and oxygen requirements. The transport and delivery of oxygen in machine perfusion are key determinants in preserving organ viability and cellular function. Oxygen delivery is more challenging than carbon dioxide removal, and oxygenation of the perfusion fluid is temperature dependent. The maximal oxygen content of water-based solutions is inversely related to the temperature, while cellular oxygen demand correlates positively with temperature. Machine perfusion above 20◦C will therefore require an oxygen carrier to enable sufficient oxygen delivery to the liver. Human red blood cells are the most physiological oxygen carriers. Alternative artificial oxygen transporters are hemoglobin-based oxygen carriers, perfluorocarbons, and an extracellular oxygen carrier derived from a marine invertebrate. We describe the principles of oxygen transport, delivery, and consumption in machine perfusion for donor livers using different oxygen carrier-based perfusion solutions and we discuss the properties, advantages, and disadvantages of these carriers and their use.
UR - http://www.scopus.com/inward/record.url?scp=85098557837&partnerID=8YFLogxK
U2 - 10.3390/ijms22010235
DO - 10.3390/ijms22010235
M3 - Review article
C2 - 33379394
AN - SCOPUS:85098557837
SN - 1661-6596
VL - 22
SP - 1
EP - 15
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 1
M1 - 235
ER -