Oxygenation measurement by multi-wavelength oxygen-dependent phosphorescence and delayed fluorescence: catchment depth and application in intact heart

Gianmarco Balestra, MCG Aalders, Patricia Specht, Can Ince, Bert Mik

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5 Citations (Scopus)

Abstract

Oxygen delivery and metabolism represent key factors for organ function in health and disease. We describe the optical key characteristics of a technique to comprehensively measure oxygen tension (PO2) in myocardium, using oxygen-dependent quenching of phosphorescence and delayed fluorescence of porphyrins, by means of Monte Carlo simulations and ex vivo experiments. Oxyphor G2 (microvascular PO2) was excited at 442 nm and 632 nm and protoporphyrin IX (mitochondrial PO2) at 510 nm. This resulted in catchment depths of 161 (86) mu m, 350 (307) mu m and 262 (255) mu m respectively, as estimated by Monte Carlo simulations and ex vivo experiments (brackets). The feasibility to detect changes in oxygenation within separate anatomical compartments is demonstrated in rat heart in vivo. [GRAPHICS] .
Original languageUndefined/Unknown
Pages (from-to)615-628
Number of pages14
JournalJournal of Biophotonics
Volume8
Issue number8
DOIs
Publication statusPublished - 2015

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