PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations

Elke Boone, On behalf of the EuroClonality consortium, Kim C. Heezen, Patricia J.T.A. Groenen, Ton Langerak

Research output: Chapter/Conference proceedingChapterAcademic

7 Citations (Scopus)

Abstract

Assessment of the presence of clonal lymphoproliferations via polymerase chain reaction (PCR)-based analysis of rearranged immunoglobulin (IG) or T-cell receptor (TR) genes is a valuable method in the diagnosis of suspect lymphoproliferative disorders. Additionally, this methodology can be used for evaluating dissemination of lymphoma cells and for studying the clonal relationship between multiple (different locations) or consecutive (over time) lymphomas. Here we describe an integrated approach to assess clonality via analysis of Ig heavy chain (IGH), Ig kappa (IGK), TCR beta (TRB), and TCR gamma (TRG) gene rearrangements, based on the standardized multiplex PCRs as originally developed by the European BIOMED-2 consortium. The described protocol covers the pre-analytical phase of DNA isolation (from formalin-fixed paraffin-embedded and fresh tissues, body fluids, peripheral blood, and bone marrow), the analytical phase of PCR GeneScan and heteroduplex analysis, and the post-analytical interpretation of the obtained profiles, following established guidelines.
Original languageEnglish
Title of host publicationLymphoma
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages77-103
Number of pages27
EditionSecond edition
ISBN (Electronic)978-1-4939-9151-8
ISBN (Print)978-1-4939-9150-1
DOIs
Publication statusPublished - 2019

Publication series

SeriesMethods in Molecular Biology
Volume1956
ISSN1064-3745

Research programs

  • EMC MM-02-72-01

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