Persistence and evolution of feline coronavirus in a closed cat-breeding colony

A. A.P.M. Herrewegh, M. Mähler, H. J. Hedrich, B. L. Haagmans, H. F. Egberink, M. C. Horzinek, P. J.M. Rottier, R. J. De Groot*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

126 Citations (Scopus)

Abstract

Feline coronavirus (FCoV) persistence and evolution were studied in a closed cat-breeding facility with an endemic serotype 1 FCoV infection. Viral RNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in the faces and/or plasma of 36 of 42 cats (86%) tested. Of 6 cats, identified as FCoV shedders during the initial survey, 4 had detectable viral RNA in the feces when tested 111 days later. To determine whether this was due to continuous reinfection or to viral persistence, 2 cats were placed in strict isolation and virus shedding in the faces was monitored every 2-4 days. In 1 of the cats, virus shedding continued for up to 7 months. The other animal was sacrificed after 124 days of continuous virus shedding in order to identify the sites of viral replication. Viral mRNA was detected only in the ileum, colon, and rectum. Also in these tissues, FCoV-infected cells were identified by immunohistochemistry. These findings provide the first formal evidence that FCoV causes chronic enteric infections. To assess FCoV heterogeneity in the breeding facility and to study viral evolution during chronic infection, FCoV quasispecies sampled from individual cats were characterized by RT-PCR amplification of selected regions of the viral genome followed by sequence analysis. Phylogenetic comparison of nucleotides 7-146 of ORF7b to corresponding sequences obtained for independent European aria American isolates indicated that the viruses in the breeding facility form a clade and are likely to have originated from a single founder infection. Comparative consensus sequence analysis of the more variable region formed by residues 79-478 of the S gene revealed that each eat harbored a distinct FCoV quasispecies. Moreover, FCoV appeared to De subject to immune selection during chronic infection. The combined data support a model in which the endemic infection is maintained by chronically infected carriers. Virtually every cat born to the breeding facility becomes infected, indicating that FCoV is sprees very efficiently. FCoV-infected cats, however, appear to resist superinfection by closely related FCoVs.

Original languageEnglish
Pages (from-to)349-363
Number of pages15
JournalVirology
Volume234
Issue number2
DOIs
Publication statusPublished - 4 Aug 1997

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