TY - JOUR
T1 - Phenotypic characterization of epidemic versus sporadic strains of methicillin-resistant Staphylococcus aureus
AU - Van Wamel, W. J.B.
AU - Fluit, A. C.
AU - Wadstrom, T.
AU - van Dijk, H.
AU - Verhoef, J.
AU - Vandenbroucke-Grauls, C. M.J.E.
PY - 1995
Y1 - 1995
N2 - Forty strains of methicillin-resistant Staphylococcus aureus (MRSA) were divided on the basis of their epidemiologic behavior into two subgroups, sporadic MRSA (SMRSA) and epidemic MRSA (EMRSA) strains. The strains were examined for binding of 125I-labelled fibronectin, vitronectin, collagen, Fc fragments of immunoglobulin G, and fibrinogen. A significant difference between EMRSA and SMRSA strains was found for binding of 125I-labelled fibrinogen and for Fc fragments of immunoglobulin G, (P < 0.05). No significant difference in the binding of 125I-labelled fibronectin and collagen was found between EMRSA and SMRSA strains. The binding of 125I- labelled vitronectin to MRSA strains was found to be aspecific. Capsular serotypes of the strains were determined with monoclonal antibodies against capsular types 5 and 8. Strains could be divided into the following four groups: types 5, 8, and 5/8 and nontypeable. More nontypeable strains were found in the EMRSA group (66.6%). Significantly more EMRSA strains (79%) than SMRSA strains (44%) produced α-toxin (P < 0.025). Logistic regression analysis using a combination of the parameters 125I-labelled immunoglobulin G binding, capsular type, and α-toxin production predicted the epidemic character with a sensitivity of 83% and a specificity of 75%.
AB - Forty strains of methicillin-resistant Staphylococcus aureus (MRSA) were divided on the basis of their epidemiologic behavior into two subgroups, sporadic MRSA (SMRSA) and epidemic MRSA (EMRSA) strains. The strains were examined for binding of 125I-labelled fibronectin, vitronectin, collagen, Fc fragments of immunoglobulin G, and fibrinogen. A significant difference between EMRSA and SMRSA strains was found for binding of 125I-labelled fibrinogen and for Fc fragments of immunoglobulin G, (P < 0.05). No significant difference in the binding of 125I-labelled fibronectin and collagen was found between EMRSA and SMRSA strains. The binding of 125I- labelled vitronectin to MRSA strains was found to be aspecific. Capsular serotypes of the strains were determined with monoclonal antibodies against capsular types 5 and 8. Strains could be divided into the following four groups: types 5, 8, and 5/8 and nontypeable. More nontypeable strains were found in the EMRSA group (66.6%). Significantly more EMRSA strains (79%) than SMRSA strains (44%) produced α-toxin (P < 0.025). Logistic regression analysis using a combination of the parameters 125I-labelled immunoglobulin G binding, capsular type, and α-toxin production predicted the epidemic character with a sensitivity of 83% and a specificity of 75%.
UR - http://www.scopus.com/inward/record.url?scp=0029069282&partnerID=8YFLogxK
U2 - 10.1128/jcm.33.7.1769-1774.1995
DO - 10.1128/jcm.33.7.1769-1774.1995
M3 - Article
C2 - 7545178
AN - SCOPUS:0029069282
SN - 0095-1137
VL - 33
SP - 1769
EP - 1774
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 7
ER -