TY - CHAP
T1 - Phosphatidylserine exposure by apoptotic cells
T2 - a phylogenetically conserved mechanism
AU - van den Eijnde, Stefan M.
AU - Boshart, Lenard
AU - Baehrecke, Eric H.
AU - Reutelingsperger, Chris P.M.
AU - Vermeij-Keers, Christl
PY - 1998
Y1 - 1998
N2 - Tolerance of the existence of the individual cell in multicellular organisms is mediated by the distribution of the various phospholipid species across the bilayer of the plasma membrane. This concept arises from in vitro studies, which show that cellsurface exposed phosphatidylserine on ageing erythrocytes and apoptotic leukocytes triggers elimination of these cells by phagocytosis. In contrast, blood cells are inert in this respect when this aminophospholipid is predominantly residing in the plasma membrane leaflet facing the cytoplasm. We have studied the in vivo distribution of cell surface-exposed phosphatidylserine by injecting biotinylated AnxV, a Ca2+dependent phosphatidylserine binding protein, into viabIe mouse and chick embryos and Drosophila pupae. The apparent binding of (Annexin V) to cells that were present in regions of developmental cell death and that were exhibiting the morphology which is characteristic of apoptosis indicates that phosphatidylserine exposure by apoptotic cells is a phylogenetically conserved mechanism.
AB - Tolerance of the existence of the individual cell in multicellular organisms is mediated by the distribution of the various phospholipid species across the bilayer of the plasma membrane. This concept arises from in vitro studies, which show that cellsurface exposed phosphatidylserine on ageing erythrocytes and apoptotic leukocytes triggers elimination of these cells by phagocytosis. In contrast, blood cells are inert in this respect when this aminophospholipid is predominantly residing in the plasma membrane leaflet facing the cytoplasm. We have studied the in vivo distribution of cell surface-exposed phosphatidylserine by injecting biotinylated AnxV, a Ca2+dependent phosphatidylserine binding protein, into viabIe mouse and chick embryos and Drosophila pupae. The apparent binding of (Annexin V) to cells that were present in regions of developmental cell death and that were exhibiting the morphology which is characteristic of apoptosis indicates that phosphatidylserine exposure by apoptotic cells is a phylogenetically conserved mechanism.
UR - https://archive.org/details/pharmaceuticalin0000unse_r8h3
M3 - Chapter
SN - 0444858237
T3 - Verhandelingen (KNAW); Afd. Natuurkunde
SP - 63
EP - 74
BT - Pharmaceutical intervention in apoptotic pathways
A2 - Nagelkerke, J.F.
A2 - van Dierendonck, J.H.
A2 - Noteborn, M.H.M.
CY - Amsterdam
ER -