Phosphodiesterase 1 regulation is a key mechanism in vascular aging

Paula K. Bautista Nino, Matej Durik, Jan Danser, René de Vries, Usha M. Musterd-Bhaggoe, Marcel Meima, Maryam Kavousi, Mohsen Ghanbari, Jan Hoeijmakers, Christopher J. O'Donnell, Nora Franceschini, Ger M.J. Janssen, Jo G.R. De Mey, Yiwen Liu, Catherine M. Shanahan, OH Franco Duran, Abbas Dehghan, Anton Roks*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Reduced nitric oxide (NO)/cGMP signalling is observed in age-related vascular disease. We hypothesize that this disturbed signalling involves effects of genomic instability, a primary causal factor in aging, on vascular smooth muscle cells (VSMCs) and that the underlying mechanism plays a role in human age-related vascular disease. To test our hypothesis, we combined experiments in mice with genomic instability resulting from the defective nucleotide excision repair gene ERCC1 (Ercc1(d/-) mice), human VSMC cultures and population genome-wide association studies (GWAS). Aortic rings of Ercc1(d/-) mice showed 43% reduced responses to the soluble guanylate cyclase (sGC) stimulator sodium nitroprusside (SNP). Inhibition of phosphodiesterase (PDE) 1 and 5 normalized SNP-relaxing effects in Ercc1(d/-) to wild-type (WT) levels. PDE1C levels were increased in lung and aorta. cGMP hydrolysis by PDE in lungs was higher in Ercc1(d/-) mice. No differences in activity or levels of cGMP-dependent protein kinase 1 or sGC were observed in Ercc1(d/-) mice compared with WT. Senescent human VSMC showed elevated PDE1A and PDE1C and PDE5 mRNA levels (11.6-, 9- and 2.3-fold respectively), which associated with markers of cellular senescence. Conversely, PDE1 inhibition lowered expression of these markers. Human genetic studies revealed significant associations of PDE1A single nucleotide polymorphisms with diastolic blood pressure (DBP; beta = 0.28, P = 2.47x10(-5)) and carotid intima-media thickness (cIMT; beta = -0.0061, P = 2.89 x 10(-5)). In summary, these results show that genomic instability and cellular senescence in VSMCs increase PDE1 expression. This might play a role in aging-related loss of vasodilator function, VSMC senescence, increased blood pressure and vascular hypertrophy.
Original languageEnglish
Pages (from-to)1061-1075
Number of pages15
JournalClinical Science
Issue number12
Publication statusPublished - 13 Oct 2015

Bibliographical note

This work was supported by the Academy of Sciences of the Czech
Republic [grant number RVO 68378050 (to M.D.)]; the BIOCEV –
Biotechnology and Biomedicine Centre of the Academy of Sciences
and Charles University [grant number CZ.1.05/1.1.00/02.0109]


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