Plasma membrane recruitment of dephosphorylated beta-catenin upon activation of the Wnt pathway

J Hendriksen, M (Marnix) Jansen, CM Brown, H te Velde, MA van Ham, Niels Galjart, GJ Offerhaus, F Fagotto, M Fornerod

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65 Citations (Scopus)

Abstract

The standard model of Wnt signaling specifies that after receipt of a Wnt ligand at the membranous receptor complex, downstream mediators inhibit a cytoplasmic destruction complex, allowing beta-catenin to accumulate in the cytosol and nucleus and co-activate Wnt target genes. Unexpectedly, shortly after Wnt treatment, we detected the dephosphorylated form of beta-catenin at the plasma membrane, where it displayed a discontinuous punctate labeling. This pool of beta-catenin could only be detected in E-cadherin(-/-) cells, because in E-cadherin(+/+) cells Wnt-induced, membranous beta-catenin was concealed by a constitutive junctional pool. Wnt-signaling-dependent dephosphorylated beta-catenin colocalized at the plasma membrane with two members of the destruction complex - APC and axin - and the activated Wnt co-receptor LRP6. beta-catenin induced through the Wnt receptor complex was significantly more competent transcriptionally than overexpressed beta-catenin, both in cultured cells and in early Xenopus embryos. Our data reveal a new step in the processing of the Wnt signal and suggest regulation of signaling output beyond the level of protein accumulation.
Original languageUndefined/Unknown
Pages (from-to)1793-1802
Number of pages10
JournalJournal of Cell Science
Volume121
Issue number11
DOIs
Publication statusPublished - 2008

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