Plasmin inhibitors in the prevention of systemic effects during thrombolytic therapy: Specific role of the plasminogen-binding form of α2-antiplasmin

Frank W.G. Leebeek*, Cornelis Kluft, Eduard A. R. Knot, Petronella Los, Adam F. Cohen, A. Jacob Six

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

To delineate the role of plasmin inhibitors, especially the two molecular forms of α2-antiplasmin (that is, the plasminogen-binding and the nonplasminogen-binding forms), in the control of systemic effects during thrombolytic therapy, the consumption of plasmin inhibitors and the degree of fibrinogen breakdown were studied in 35 patients with acute myocardial infarction treated with recombinant tissue-type plasminogen activator (rt-PA) or streptokinase. At a low degree of plasminogen activation (in six patients treated with rt-PA), plasminogen-binding α2-antiplasmin was consumed first. At a higher degree of plasminogen activation (in 20 patients), plasminogenbinding α2-antiplasmin became exhausted (<20%) and other plasmin inhibitors (that is, nonplasminogen-binding α2-antiplasmin and α2-macroglobulin) were consumed. After extensive plasminogen activation (in nine patients treated with streptokinase), plasminogen-binding α2-antiplasmin consumption was complete and nonplasminogen-binding α2-antiplasmin and α2-macroglobulin were consumed to about 30% to 50% of the pretreatment level. No significant C1-inactivator consumption occurred, even at extreme degrees of plasminogen activation. Fibrinogen breakdown as a marker for systemic effects correlated strongly with consumption of plasminogenbinding α2-antiplasmin. Fibrinogen breakdown did occur, but only when the amount of plasminogen-binding α2-antiplasmin was decreased to <20% of the pretreatment level. The other plasmin inhibitors could not prevent fibrinogen breakdown. These results were confirmed by in vitro studies. It is concluded that plasminogen-binding α2-antiplasmin is the most important inhibitor of plasmin in the circulation. To obtain systemic effects, the amount of plasminogen-binding α2-antiplasmin, which is a threshold in the circulation, has to be overcome. This indicates that plasminogen-binding α2-antiplasmin may be a new marker to predict systemic effects during thrombolytic therapy.

Original languageEnglish
Pages (from-to)1212-1220
Number of pages9
JournalJournal of the American College of Cardiology
Volume15
Issue number6
DOIs
Publication statusPublished - 1990
Externally publishedYes

Bibliographical note

Copyright © 1990 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.

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