Pleurostomophora ochracea, a Novel Agent of Human Eumycetoma with Yellow Grains

NA Mhmoud, SA Ahmed, AH Fahal, GS de Hoog, AHGG van den Ende, Wendy Laureijssen - van de Sande

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Abstract

The first yellow-grain fungal mycetoma, in a 60-year-old man from Central Sudan, is reported. Morphological and phylogenetic analysis of the ribosomal small subunit (SSU), large subunit (LSU), internal transcribed spacer (ITS), beta-tubulin (BT2), actin (ACT1), and elongation factor (TEF1) genes revealed that the isolate deviated from any known agent of mycetoma; it clustered in the genus Pleurostoma (anamorph genus, Pleurostomophora) in the order Calosphaeriales. The novel species, here named Pleurostomophora ochracea, is characterized by phenotypic features. The species proved to be highly susceptible to itraconazole, ketoconazole, posaconazole, and voriconazole, but not to fluconazole. The fungus was inhibited by caspofungin at 8 mu g/ml, while no inhibition was found with 5-flucytosine (MIC > 64 mu g/ml). Compared to other members of the genus Pleurostomophora, P. ochracea is slow growing, with a relatively high optimum growth temperature (36 to 37 degrees C). This is the first case of a yellow-grain fungal mycetoma; yellow grains are otherwise of bacterial nature. Our case emphasizes that identification of mycetoma agents by the color of the grain only is not sufficient and may lead to inappropriate therapy.
Original languageUndefined/Unknown
Pages (from-to)2987-2994
Number of pages8
JournalJournal of Clinical Microbiology
Volume50
Issue number9
DOIs
Publication statusPublished - 2012

Research programs

  • EMC MM-04-28-01

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