Pneumococcal conjugate vaccines

Carla C.A.M. Peeters*, Anne Marie Tenbergen-Meekes, Bart Haagmans, Dolf Evenberg, Jan T. Poolman, Ben J.M. Zegers, Ger T. Rijkers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

We have prepared conjugates of pneumococcal type 4 polysaccharides (PS4) or oligosaccharides to tetanus toxoid using the carbodiimide method. The use of a spacer, 6-aminohexanoic acid, resulted in higher incorporation of carrier protein. Conjugates contained up to 10% free polysaccharide, but no free protein. In general, polysaccharide conjugates induced higher anti-PS4 IgG antibody titers than oligosaccharide conjugates. Conjugates with the highest amount of incorporated protein were the most immunogenic. The response to conjugated PS4 does show characteristics of a T cell-dependent antibody response, in terms of both isotype distribution and induction of immunological memory. Repeated immunization with high doses of PS4TT conjugate resulted in a virtually negative anti-PS4 IgG response, suggestive of the induction of high dose tolerance.

Original languageEnglish
Pages (from-to)267-274
Number of pages8
JournalImmunology Letters
Volume30
Issue number2
DOIs
Publication statusPublished - Oct 1991
Externally publishedYes

Bibliographical note

Copyright © 1991 Published by Elsevier B.V

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