Pneumococcal conjugate vaccines

Carla C.A.M. Peeters; Anne Marie Tenbergen-Meekes; Bart Haagmans; Dolf Evenberg; Jan T. Poolman; Ben J.M. Zegers; Ger T. Rijkers

doi:10.1016/0165-2478(91)90036-A

Pneumococcal conjugate vaccines

Carla C.A.M. Peeters^*, Anne Marie Tenbergen-Meekes, Bart Haagmans, Dolf Evenberg, Jan T. Poolman, Ben J.M. Zegers, Ger T. Rijkers

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

24 Citations (Scopus)

Abstract

We have prepared conjugates of pneumococcal type 4 polysaccharides (PS4) or oligosaccharides to tetanus toxoid using the carbodiimide method. The use of a spacer, 6-aminohexanoic acid, resulted in higher incorporation of carrier protein. Conjugates contained up to 10% free polysaccharide, but no free protein. In general, polysaccharide conjugates induced higher anti-PS4 IgG antibody titers than oligosaccharide conjugates. Conjugates with the highest amount of incorporated protein were the most immunogenic. The response to conjugated PS4 does show characteristics of a T cell-dependent antibody response, in terms of both isotype distribution and induction of immunological memory. Repeated immunization with high doses of PS4TT conjugate resulted in a virtually negative anti-PS4 IgG response, suggestive of the induction of high dose tolerance.

Original language	English
Pages (from-to)	267-274
Number of pages	8
Journal	Immunology Letters
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(91)90036-A
Publication status	Published - Oct 1991
Externally published	Yes

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10.1016/0165-2478(91)90036-A

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title = "Pneumococcal conjugate vaccines",

abstract = "We have prepared conjugates of pneumococcal type 4 polysaccharides (PS4) or oligosaccharides to tetanus toxoid using the carbodiimide method. The use of a spacer, 6-aminohexanoic acid, resulted in higher incorporation of carrier protein. Conjugates contained up to 10% free polysaccharide, but no free protein. In general, polysaccharide conjugates induced higher anti-PS4 IgG antibody titers than oligosaccharide conjugates. Conjugates with the highest amount of incorporated protein were the most immunogenic. The response to conjugated PS4 does show characteristics of a T cell-dependent antibody response, in terms of both isotype distribution and induction of immunological memory. Repeated immunization with high doses of PS4TT conjugate resulted in a virtually negative anti-PS4 IgG response, suggestive of the induction of high dose tolerance.",

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T1 - Pneumococcal conjugate vaccines

AU - Peeters, Carla C.A.M.

AU - Tenbergen-Meekes, Anne Marie

AU - Haagmans, Bart

AU - Evenberg, Dolf

AU - Poolman, Jan T.

AU - Zegers, Ben J.M.

AU - Rijkers, Ger T.

PY - 1991/10

Y1 - 1991/10

N2 - We have prepared conjugates of pneumococcal type 4 polysaccharides (PS4) or oligosaccharides to tetanus toxoid using the carbodiimide method. The use of a spacer, 6-aminohexanoic acid, resulted in higher incorporation of carrier protein. Conjugates contained up to 10% free polysaccharide, but no free protein. In general, polysaccharide conjugates induced higher anti-PS4 IgG antibody titers than oligosaccharide conjugates. Conjugates with the highest amount of incorporated protein were the most immunogenic. The response to conjugated PS4 does show characteristics of a T cell-dependent antibody response, in terms of both isotype distribution and induction of immunological memory. Repeated immunization with high doses of PS4TT conjugate resulted in a virtually negative anti-PS4 IgG response, suggestive of the induction of high dose tolerance.

AB - We have prepared conjugates of pneumococcal type 4 polysaccharides (PS4) or oligosaccharides to tetanus toxoid using the carbodiimide method. The use of a spacer, 6-aminohexanoic acid, resulted in higher incorporation of carrier protein. Conjugates contained up to 10% free polysaccharide, but no free protein. In general, polysaccharide conjugates induced higher anti-PS4 IgG antibody titers than oligosaccharide conjugates. Conjugates with the highest amount of incorporated protein were the most immunogenic. The response to conjugated PS4 does show characteristics of a T cell-dependent antibody response, in terms of both isotype distribution and induction of immunological memory. Repeated immunization with high doses of PS4TT conjugate resulted in a virtually negative anti-PS4 IgG response, suggestive of the induction of high dose tolerance.

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U2 - 10.1016/0165-2478(91)90036-A

DO - 10.1016/0165-2478(91)90036-A

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JO - Immunology Letters

JF - Immunology Letters

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Pneumococcal conjugate vaccines

Abstract

Bibliographical note

UN SDGs

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