Pre-B-cell leukemias in Btk/Slp65-deficient mice arise independently of ongoing V(D)J recombination activity

Van Ta, AB (Aalzen) de Haan, Marjolein De Jong - de Bruijn, Gemma Dingjan, Rudi Hendriks

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)


The adapter protein Slp65 and Bruton's tyrosine kinase (Btk) are key components of the precursor-B (pre-B) cell receptor (pre-BCR) signaling pathway. Slp65-deficient mice spontaneously develop pre-B-cell leukemia, expressing high levels of the pre-BCR on their cell surface. As leukemic Slp65-deficient pre-B cells express the recombination activating genes (Rag) 1 and Rag2, and manifest ongoing immunoglobulin (Ig) light-chain rearrangement, it has been hypothesized that deregulated recombinase activity contributes to malignant transformation. In this report, we investigated whether Rag-induced DNA damage is involved in oncogenic transformation of Slp65-deficient B cells. We employed Btk/Slp65 double-deficient mice carrying an autoreactive 3-83 mu delta BCR transgene. When developing B cells in their bone marrow express this BCR, the V(D)J recombination machinery will be activated, allowing for secondary Ig light-chain gene rearrangements to occur. This phenomenon, called receptor editing, will rescue autoreactive B cells from apoptosis. We observed that 3-83 mu delta transgenic Btk/Slp65 double-deficient mice developed B-cell leukemias expressing both the 3-83 mu delta BCR and the pre-BCR components lambda 5/VpreB. Importantly, such leukemias were found at similar frequencies in mice concomitantly deficient for Rag1 or the non-homologous end-joining factor DNA-PKcs. We therefore conclude that malignant transformation of Btk/Slp65 double-deficient pre-B cells is independent of deregulated V(D) J recombination activity. Leukemia (2011) 25, 48-56; doi: 10.1038/leu.2010.246; published online 29 October 2010
Original languageUndefined/Unknown
Pages (from-to)48-56
Number of pages9
Issue number1
Publication statusPublished - 2011

Cite this