Preconception folic acid use influences the follicle fluid proteome

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BackgroundThe investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for invitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular metabolism and oocyte and embryo quality. From this background, we aim to identify a folate-related follicle fluid proteome that associates with IVF/ICSI treatment outcome. MethodsIn a nested case-control study embedded in a periconception cohort, we performed qualitative and quantitative proteomic analyses using nanoflow LC-MS/MS and TMT labelling in 30 monofollicular fluid samples from women undergoing IVF/ICSI treatment of which 15 used and 15 did not use a folic acid supplement. The protein data are analysed using scaffold proteome Software and differential abundances are expressed as Log2-fold change. Blood samples were obtained before and after treatment for determination of biomarkers of 1-C metabolism and estradiol. ResultsWe identified 227 uniquely expressed proteins in follicular fluid. In folic acid supplement users compared to nonusers, we established a lower abundance of C-reactive protein (-203; P=<001) and higher abundances of apolipoproteins from high-density lipoprotein (HDL), most notably A-I (+128; P=<001) and C-I (+111; P=0016). ConclusionPreconception folic acid supplement use is associated with suppression of the inflammatory pathway and upregulation of the HDL pathway in human follicular fluid, being a preferential source of cholesterol for steroid hormone synthesis. Studies are needed on the tissue specificity and on the beneficial effects of embryo quality and IVF/ICSI treatment outcome of the proteome of these pathways.
Original languageUndefined/Unknown
Pages (from-to)833-841
Number of pages9
JournalEuropean Journal of Clinical Investigation
Issue number8
Publication statusPublished - 2015

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