Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis

Corina C P M Bens; Andreas Voss; Corné H W Klaassen

doi:10.1128/JCM.44.5.1875-1876.2006

Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis

Corina C P M Bens
, Andreas Voss
, Corné H W Klaassen

Canisius Wilhelmina Hospital

Research output: Contribution to journal › Article › Academic › peer-review

123 Citations (Scopus)

Abstract

Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.

Original language	English
Pages (from-to)	1875-6
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1128/JCM.44.5.1875-1876.2006
Publication status	Published - May 2006
Externally published	Yes

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10.1128/JCM.44.5.1875-1876.2006

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title = "Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis",

abstract = "Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.",

author = "Bens, \{Corina C P M\} and Andreas Voss and Klaassen, \{Corn{\'e} H W\}",

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Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis. / Bens, Corina C P M; Voss, Andreas; Klaassen, Corné H W.
In: Journal of Clinical Microbiology, Vol. 44, No. 5, 05.2006, p. 1875-6.

Research output: Contribution to journal › Article › Academic › peer-review

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AB - Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.

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DO - 10.1128/JCM.44.5.1875-1876.2006

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