Presentation of chemokine SDF-1α by fibronectin mediates directed migration of T cells

A. J. Pelletier; L. J.W. Van der Laan; P. Hildbrand; M. A. Siani; D. A. Thompson; P. E. Dawson; B. E. Torbett; D. R. Salomon

doi:10.1182/blood.v96.8.2682

Presentation of chemokine SDF-1α by fibronectin mediates directed migration of T cells

A. J. Pelletier
, L. J.W. Van der Laan
, P. Hildbrand
, M. A. Siani
, D. A. Thompson
, P. E. Dawson
, B. E. Torbett
, D. R. Salomon^*

^*Corresponding author for this work

Scripps Research Institute

Research output: Contribution to journal › Article › Academic › peer-review

120 Citations (Scopus)

Abstract

The role of chemokine - matrix interactions in integrin-dependent T-cell migration was examined to address the critical question of how chemokines provide directional information. The chemokine SDF-1α binds fibronectin (Fn) with a low nanomolar K_d (equilibrium dissociation constant). SDF-1α presented by Fn induced directed migration. Spatial concentration gradients of chemokine were not required to maintain directed migration. Fn-presented chemokine induced the polarization of cells, including the redistribution of the SDF-1α receptor, to the basal surface and leading edge of the cell. A new model for directed migration is proposed in which the co-presentation of an adhesive matrix and chemokine provides the necessary positional information independent of a soluble spatial gradient.

Original language	English
Pages (from-to)	2682-2690
Number of pages	9
Journal	Blood
Volume	96
Issue number	8
DOIs	https://doi.org/10.1182/blood.v96.8.2682
Publication status	Published - 15 Oct 2000
Externally published	Yes

Bibliographical note

© 2000 by The American Society of Hematology

Supported by National Institutes of Health (NIH) grants AI42384 and GM53489, Bethesda, MD, and a grant from the Katherine Huber-Steiner Foundation, Bern, Switzerland

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10.1182/blood.v96.8.2682

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title = "Presentation of chemokine SDF-1α by fibronectin mediates directed migration of T cells",

abstract = "The role of chemokine - matrix interactions in integrin-dependent T-cell migration was examined to address the critical question of how chemokines provide directional information. The chemokine SDF-1α binds fibronectin (Fn) with a low nanomolar Kd (equilibrium dissociation constant). SDF-1α presented by Fn induced directed migration. Spatial concentration gradients of chemokine were not required to maintain directed migration. Fn-presented chemokine induced the polarization of cells, including the redistribution of the SDF-1α receptor, to the basal surface and leading edge of the cell. A new model for directed migration is proposed in which the co-presentation of an adhesive matrix and chemokine provides the necessary positional information independent of a soluble spatial gradient. ",

author = "Pelletier, \{A. J.\} and \{Van der Laan\}, \{L. J.W.\} and P. Hildbrand and Siani, \{M. A.\} and Thompson, \{D. A.\} and Dawson, \{P. E.\} and Torbett, \{B. E.\} and Salomon, \{D. R.\}",

note = "{\textcopyright} 2000 by The American Society of Hematology Supported by National Institutes of Health (NIH) grants AI42384 and GM53489, Bethesda, MD, and a grant from the Katherine Huber-Steiner Foundation, Bern, Switzerland",

year = "2000",

month = oct,

day = "15",

doi = "10.1182/blood.v96.8.2682",

language = "English",

volume = "96",

pages = "2682--2690",

journal = "Blood",

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TY - JOUR

T1 - Presentation of chemokine SDF-1α by fibronectin mediates directed migration of T cells

AU - Pelletier, A. J.

AU - Van der Laan, L. J.W.

AU - Hildbrand, P.

AU - Siani, M. A.

AU - Thompson, D. A.

AU - Dawson, P. E.

AU - Torbett, B. E.

AU - Salomon, D. R.

N1 - © 2000 by The American Society of Hematology Supported by National Institutes of Health (NIH) grants AI42384 and GM53489, Bethesda, MD, and a grant from the Katherine Huber-Steiner Foundation, Bern, Switzerland

PY - 2000/10/15

Y1 - 2000/10/15

N2 - The role of chemokine - matrix interactions in integrin-dependent T-cell migration was examined to address the critical question of how chemokines provide directional information. The chemokine SDF-1α binds fibronectin (Fn) with a low nanomolar Kd (equilibrium dissociation constant). SDF-1α presented by Fn induced directed migration. Spatial concentration gradients of chemokine were not required to maintain directed migration. Fn-presented chemokine induced the polarization of cells, including the redistribution of the SDF-1α receptor, to the basal surface and leading edge of the cell. A new model for directed migration is proposed in which the co-presentation of an adhesive matrix and chemokine provides the necessary positional information independent of a soluble spatial gradient.

AB - The role of chemokine - matrix interactions in integrin-dependent T-cell migration was examined to address the critical question of how chemokines provide directional information. The chemokine SDF-1α binds fibronectin (Fn) with a low nanomolar Kd (equilibrium dissociation constant). SDF-1α presented by Fn induced directed migration. Spatial concentration gradients of chemokine were not required to maintain directed migration. Fn-presented chemokine induced the polarization of cells, including the redistribution of the SDF-1α receptor, to the basal surface and leading edge of the cell. A new model for directed migration is proposed in which the co-presentation of an adhesive matrix and chemokine provides the necessary positional information independent of a soluble spatial gradient.

UR - https://www.scopus.com/pages/publications/0034667632

U2 - 10.1182/blood.v96.8.2682

DO - 10.1182/blood.v96.8.2682

M3 - Article

C2 - 11023498

AN - SCOPUS:0034667632

SN - 0006-4971

VL - 96

SP - 2682

EP - 2690

JO - Blood

JF - Blood

IS - 8

ER -

Presentation of chemokine SDF-1α by fibronectin mediates directed migration of T cells

Abstract

Bibliographical note

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