TY - JOUR
T1 - Profiling of humoral immune responses to norovirus in children across Europe
AU - Villabruna, Nele
AU - Izquierdo-Lara, Ray W.
AU - Schapendonk, Claudia M.E.
AU - de Bruin, Erwin
AU - Chandler, Felicity
AU - Thao, Tran Thi Nhu
AU - Westerhuis, Brenda M.
AU - van Beek, Janko
AU - Sigfrid, Louise
AU - Giaquinto, Carlo
AU - Goossens, Herman
AU - Bielicki, Julia A.
AU - Kohns Vasconcelos, Malte
AU - Fraaij, Pieter L.A.
AU - Koopmans, Marion P.G.
AU - de Graaf, Miranda
N1 - Funding Information:
The authors gratefully acknowledge the submitting laboratories of the sequences from the NoroNet database. This work was supported by the Erasmus MC Grant mRACE and the European Union’s Horizon 2020 Grant VEO (Grant No. 874735), the ZonMW TOP Project (91213058), and the PREPARE Europe (EU FP7 Grant No. 602525).
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/8/22
Y1 - 2022/8/22
N2 - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.
AB - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.
UR - http://www.scopus.com/inward/record.url?scp=85136222184&partnerID=8YFLogxK
U2 - 10.1038/s41598-022-18383-6
DO - 10.1038/s41598-022-18383-6
M3 - Article
C2 - 35995986
AN - SCOPUS:85136222184
SN - 2045-2322
VL - 12
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 14275
ER -
