Profiling of humoral immune responses to norovirus in children across Europe

Nele Villabruna; Ray W. Izquierdo-Lara; Claudia M.E. Schapendonk; Erwin de Bruin; Felicity Chandler; Tran Thi Nhu Thao; Brenda M. Westerhuis; Janko van Beek; Louise Sigfrid; Carlo Giaquinto; Herman Goossens; Julia A. Bielicki; Malte Kohns Vasconcelos; Pieter L.A. Fraaij; Marion P.G. Koopmans; Miranda de Graaf

doi:10.1038/s41598-022-18383-6

Profiling of humoral immune responses to norovirus in children across Europe

Nele Villabruna
, Ray W. Izquierdo-Lara
, Claudia M.E. Schapendonk
, Erwin de Bruin
, Felicity Chandler
, Tran Thi Nhu Thao
, Brenda M. Westerhuis
, Janko van Beek
, Louise Sigfrid
, Carlo Giaquinto
, Herman Goossens
, Julia A. Bielicki
, Malte Kohns Vasconcelos
, Pieter L.A. Fraaij
, Marion P.G. Koopmans
, Miranda de Graaf^*

^*Corresponding author for this work

Federal Food Safety and Veterinary Office
University of Bern
Hebrew SeniorLife
University of Oxford
Azienda Ospedale - Università Padova
University of Antwerp
City St George's, University of London
University of Basel
Heinrich Heine University Düsseldorf

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.

Original language	English
Article number	14275
Journal	Scientific Reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-18383-6
Publication status	Published - 22 Aug 2022

Bibliographical note

Funding Information:
The authors gratefully acknowledge the submitting laboratories of the sequences from the NoroNet database. This work was supported by the Erasmus MC Grant mRACE and the European Union’s Horizon 2020 Grant VEO (Grant No. 874735), the ZonMW TOP Project (91213058), and the PREPARE Europe (EU FP7 Grant No. 602525).

Publisher Copyright:
© 2022, The Author(s).

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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Profiling of humoral immune responses to norovirus in children across EuropeFinal published version, 3.42 MBLicence: CC BY

Cite this

Villabruna, N., Izquierdo-Lara, R. W., Schapendonk, C. M. E., de Bruin, E., Chandler, F., Thao, T. T. N., Westerhuis, B. M., van Beek, J., Sigfrid, L., Giaquinto, C., Goossens, H., Bielicki, J. A., Kohns Vasconcelos, M., Fraaij, P. L. A., Koopmans, M. P. G., & de Graaf, M. (2022). Profiling of humoral immune responses to norovirus in children across Europe. Scientific Reports, 12(1), Article 14275. https://doi.org/10.1038/s41598-022-18383-6

@article{a87f2bffedff460782e764c2926be758,

title = "Profiling of humoral immune responses to norovirus in children across Europe",

abstract = "Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.",

author = "Nele Villabruna and Izquierdo-Lara, \{Ray W.\} and Schapendonk, \{Claudia M.E.\} and \{de Bruin\}, Erwin and Felicity Chandler and Thao, \{Tran Thi Nhu\} and Westerhuis, \{Brenda M.\} and \{van Beek\}, Janko and Louise Sigfrid and Carlo Giaquinto and Herman Goossens and Bielicki, \{Julia A.\} and \{Kohns Vasconcelos\}, Malte and Fraaij, \{Pieter L.A.\} and Koopmans, \{Marion P.G.\} and \{de Graaf\}, Miranda",

note = "Funding Information: The authors gratefully acknowledge the submitting laboratories of the sequences from the NoroNet database. This work was supported by the Erasmus MC Grant mRACE and the European Union{\textquoteright}s Horizon 2020 Grant VEO (Grant No. 874735), the ZonMW TOP Project (91213058), and the PREPARE Europe (EU FP7 Grant No. 602525). Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = aug,

day = "22",

doi = "10.1038/s41598-022-18383-6",

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Villabruna, N, Izquierdo-Lara, RW , Schapendonk, CME, de Bruin, E, Chandler, F, Thao, TTN, Westerhuis, BM, van Beek, J, Sigfrid, L, Giaquinto, C, Goossens, H, Bielicki, JA, Kohns Vasconcelos, M, Fraaij, PLA , Koopmans, MPG & de Graaf, M 2022, 'Profiling of humoral immune responses to norovirus in children across Europe', Scientific Reports, vol. 12, no. 1, 14275. https://doi.org/10.1038/s41598-022-18383-6

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T1 - Profiling of humoral immune responses to norovirus in children across Europe

AU - Villabruna, Nele

AU - Izquierdo-Lara, Ray W.

AU - Schapendonk, Claudia M.E.

AU - de Bruin, Erwin

AU - Chandler, Felicity

AU - Thao, Tran Thi Nhu

AU - Westerhuis, Brenda M.

AU - van Beek, Janko

AU - Sigfrid, Louise

AU - Giaquinto, Carlo

AU - Goossens, Herman

AU - Bielicki, Julia A.

AU - Kohns Vasconcelos, Malte

AU - Fraaij, Pieter L.A.

AU - Koopmans, Marion P.G.

AU - de Graaf, Miranda

N1 - Funding Information: The authors gratefully acknowledge the submitting laboratories of the sequences from the NoroNet database. This work was supported by the Erasmus MC Grant mRACE and the European Union’s Horizon 2020 Grant VEO (Grant No. 874735), the ZonMW TOP Project (91213058), and the PREPARE Europe (EU FP7 Grant No. 602525). Publisher Copyright: © 2022, The Author(s).

PY - 2022/8/22

Y1 - 2022/8/22

N2 - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.

AB - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.

UR - https://www.scopus.com/pages/publications/85136222184

U2 - 10.1038/s41598-022-18383-6

DO - 10.1038/s41598-022-18383-6

M3 - Article

C2 - 35995986

AN - SCOPUS:85136222184

SN - 2045-2322

VL - 12

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 14275

ER -

Profiling of humoral immune responses to norovirus in children across Europe

Abstract

Bibliographical note

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