Protein 4.1R binds to CLASP2 and regulates dynamics, organization and attachment of microtubules to the cell cortex

Ana Ruiz-Saenz, Jeffrey van Haren, C Laura Sayas, Laura Rangel, Jeroen Demmers, Jaime Millán, Miguel A Alonso, Niels Galjart*, Isabel Correas*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)


The microtubule (MT) cytoskeleton is essential for many cellular processes, including cell polarity and migration. Cortical platforms, formed by a subset of MT plus-end-tracking proteins, such as CLASP2, and non-MT binding proteins such as LL5β, attach distal ends of MTs to the cell cortex. However, the mechanisms involved in organizing these platforms have not yet been described in detail. Here we show that 4.1R, a FERM-domain-containing protein, interacts and colocalizes with cortical CLASP2 and is required for the correct number and dynamics of CLASP2 cortical platforms. Protein 4.1R also controls binding of CLASP2 to MTs at the cell edge by locally altering GSK3 activity. Furthermore, in 4.1R-knockdown cells MT plus-ends were maintained for longer in the vicinity of cell edges, but instead of being tethered to the cell cortex, MTs continued to grow, bending at cell margins and losing their radial distribution. Our results suggest a previously unidentified role for the scaffolding protein 4.1R in locally controlling CLASP2 behavior, CLASP2 cortical platform turnover and GSK3 activity, enabling correct MT organization and dynamics essential for cell polarity.

Original languageEnglish
Pages (from-to)4589-4601
Number of pages13
JournalJournal of Cell Science
Issue number20
Publication statusPublished - Oct 2013

Bibliographical note

This work was supported by the Ministerio de Ciencia e Innovacio´n
[grant numbers BFU2011-22859 to I.C., CSD2009-00016 and
BFU2012-32532 to M.A.A., SAF2011-22624 to J.M.]; the
Comunidad de Madrid [grant number S2010/BMD-2305 to I.C.];
the Netherlands Organization for Scientific Research [grant number
91208002 to N.G.]; and the Cancer Genomics Centre of the
Netherlands [grant number 050-060-206 to N.G.].

Research programs

  • EMC MGC-02-13-02
  • EMC MGC-02-21-01


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