Proteomic analysis of the microenvironment of developing oocytes

John Twigt; Régine Steegers - Theunissen; Karel Bezstarosti; Jeroen Demmers

doi:10.1002/pmic.201100240

Proteomic analysis of the microenvironment of developing oocytes

John Twigt, Régine Steegers - Theunissen, Karel Bezstarosti, Jeroen Demmers

Research output: Contribution to journal › Article › Academic › peer-review

52 Citations (Scopus)

Abstract

We utilized a setup based on extensive pre-fractionation of proteolytic peptides and nanoflow reversed-phase LC-MS/MS to identify the (sub) proteome of human follicular fluid (FF). In this in-depth screen, 246 specific proteins were identified, the majority of which are involved in coagulation-and immune-response pathways. Our aim is to define a set of FF protein-markers, which could predict oocyte quality.

Original language	Undefined/Unknown
Pages (from-to)	1463-1471
Number of pages	9
Journal	Proteomics
Volume	12
Issue number	9
DOIs	https://doi.org/10.1002/pmic.201100240
Publication status	Published - 2012

Research programs

EMC MGC-02-21-01
EMC MGC-02-96-01
EMC MM-01-52-07

Access to Document

10.1002/pmic.201100240

http://hdl.handle.net/1765/72247

Cite this

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