Proteomics Based Identification of Proteins with Deregulated Expression in B Cell Lymphomas

Rui Wu, Marcel Nijland, Bea Rutgers, Rianne Veenstra, Myra Langendonk, Lotte E van der Meeren, Philip M Kluin, Guanwu Li, Arjan Diepstra, Jen-Fu Chiu, Anke van den Berg, Lydia Visser*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
3 Downloads (Pure)

Abstract

Follicular lymphoma and diffuse large B cell lymphomas comprise the main entities of adult B cell malignancies. Although multiple disease driving gene aberrations have been identified by gene expression and genomic studies, only a few studies focused at the protein level. We applied 2 dimensional gel electrophoresis to compare seven GC B cell non Hodgkin lymphoma (NHL) cell lines with a lymphoblastoid cell line (LCL). An average of 130 spots were at least two folds different in intensity between NHL cell lines and the LCL. We selected approximately 38 protein spots per NHL cell line and linked them to 145 unique spots based on the location in the gel. 34 spots that were found altered in at least three NHL cell lines when compared to LCL, were submitted for LC-MS/MS. This resulted in 28 unique proteins, a substantial proportion of these proteins were involved in cell motility and cell metabolism. Loss of expression of B2M, and gain of expression of PRDX1 and PPIA was confirmed in the cell lines and primary lymphoma tissue. Moreover, inhibition of PPIA with cyclosporine A blocked cell growth of the cell lines, the effect size was associated with the PPIA expression levels. In conclusion, we identified multiple differentially expressed proteins by 2-D proteomics, and showed that some of these proteins might play a role in the pathogenesis of NHL.

Original languageEnglish
Pages (from-to)e0146624
JournalPLoS ONE
Volume11
Issue number1
DOIs
Publication statusPublished - 2016
Externally publishedYes

Bibliographical note

Funding:
RW is funded by a Abel Tasman Talent
scholarship from the University of Groningen, and
received funding from the “de Cock Foundation” for
this study. LEM is funded by a Dutch Cancer Society
fellowship. The funders had no role in study design,
data collection and analysis, decision to publish, or
preparation of the manuscript.

RW is funded by a Abel Tasman Talent scholarship from the University of Groningen, and
received funding from the “de Cock Foundation” for this study. LEM is funded by a Dutch
Cancer Society fellowship

Fingerprint

Dive into the research topics of 'Proteomics Based Identification of Proteins with Deregulated Expression in B Cell Lymphomas'. Together they form a unique fingerprint.

Cite this