Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker

Saskia du Pré, Mickey Konings, Dorenda Schoorl, Ahmed Fahal, Mark Arentshorst, Arthur Ram, Wendy van de Sande*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Madurella mycetomatis is the main cause of mycetoma, a chronic granulomatous infection for which currently no adequate therapy is available. To improve therapy, more knowledge on a molecular level is required to understand how M. mycetomatis is able to cause this disease. However, the genetic toolbox for M. mycetomatis is limited. To date, no method is available to genetically modify M. mycetomatis. In this paper, a protoplast-mediated transformation protocol was successfully developed for this fungal species, using hygromycin as a selection marker. Furthermore, using this method, a cytoplasmic-GFP-expressing M. mycetomatis strain was created. The reported methodology will be invaluable to explore the pathogenicity of M. mycetomatis and to develop reporter strains which can be useful in drug discovery as well as in genetic studies.
Original languageEnglish
Article numbere0012092
Pages (from-to)e0012092
JournalPLoS Neglected Tropical Diseases
Volume18
Issue number4
DOIs
Publication statusPublished - 5 Apr 2024

Bibliographical note

Publisher Copyright:
© 2024 du Pré et al.

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