Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography

Edwin Veldhuis Kroeze; Geert Amerongen; Marcel Dijkshoorn; JH Simon; L (Leon) van der Waal; Ieneke Hartmann; Gabriel Krestin; Thijs Kuiken; Ab Osterhaus; Koert Stittelaar

doi:10.1099/vir.0.032805-0

Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography

Edwin Veldhuis Kroeze, Geert Amerongen, Marcel Dijkshoorn, JH Simon, L (Leon) van der Waal, Ieneke Hartmann, Gabriel Krestin, Thijs Kuiken, Ab Osterhaus, Koert Stittelaar

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.

Original language	Undefined/Unknown
Pages (from-to)	1854-1858
Number of pages	5
Journal	Journal of General Virology
Volume	92
DOIs	https://doi.org/10.1099/vir.0.032805-0
Publication status	Published - 2011

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EMC MM-04-27-01
EMC NIHES-03-30-01

Access to Document

10.1099/vir.0.032805-0

http://hdl.handle.net/1765/33335

Cite this

Veldhuis Kroeze, E., Amerongen, G., Dijkshoorn, M., Simon, JH., van der Waal, L., Hartmann, I., Krestin, G., Kuiken, T., Osterhaus, A., & Stittelaar, K. (2011). Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography. Journal of General Virology, 92, 1854-1858. https://doi.org/10.1099/vir.0.032805-0

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abstract = "We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.",

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Veldhuis Kroeze, E, Amerongen, G, Dijkshoorn, M, Simon, JH, van der Waal, L, Hartmann, I, Krestin, G , Kuiken, T, Osterhaus, A & Stittelaar, K 2011, 'Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography', Journal of General Virology, vol. 92, pp. 1854-1858. https://doi.org/10.1099/vir.0.032805-0

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AU - Veldhuis Kroeze, Edwin

AU - Amerongen, Geert

AU - Dijkshoorn, Marcel

AU - Simon, JH

AU - van der Waal, L (Leon)

AU - Hartmann, Ieneke

AU - Krestin, Gabriel

AU - Kuiken, Thijs

AU - Osterhaus, Ab

AU - Stittelaar, Koert

PY - 2011

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AB - We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.

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