Quantitative analysis of myofilament protein phosphorylation in small cardiac biopsies

Ruud Zaremba, Daphne Merkus, Nazha Hamdani, Jos M J Lamers, Walter J Paulus, Cris Dos Remedios, Dirk J Duncker, Ger J M Stienen, Jolanda van der Velden*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

62 Citations (Scopus)

Abstract

Phosphorylation of cardiac myofilament proteins represents one of the main post-translational mechanisms that regulate cardiac pump function. Human studies are often limited by the amount of available tissue as biopsies taken during cardiac catheterization weigh only 1 mg (dry weight). Similarly, investigation of time- (or dose-) dependent changes in protein phosphorylation in animal studies is often hampered by tissue availability. The present study describes quantitative analysis of phosphorylation status of multiple myofilament proteins by 2-DE and Pro-Q® Diamond stained gradient gels using minor amounts (˜0.5 mg dry weight) of human and pig cardiac tissue.

Original languageEnglish
Pages (from-to)1285-90
Number of pages6
JournalProteomics - Clinical Applications
Volume1
Issue number10
DOIs
Publication statusPublished - 9 Oct 2007

Bibliographical note

Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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